Vasopressin responses in electrically coupled A7r5 cells

Ludwig Missiaen, Masahiro Oike, Martin D. Bootman, Humbert De Smedt, Jan B. Parys, Rik Casteels

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Abstract

Changes in cytosolic Ca2+ concentration ([Ca2+]i) and in membrane potential were monitored in single A7r5 smooth-muscle cells during spontaneous spiking and after arginine vasopressin stimulation. Spontaneous Ca2+ oscillations, which were associated with the occurrence of action potentials, occurred in about 90% of the confluent monolayers investigated. This spontaneous activity was synchronized amongst all the cells of the monolayer, indicating that the cells were electrically coupled. Arginine vasopressin stimulation produced a [Ca2+]i rise that was about 5 times higher than the amplitude of the spontaneous Ca2+ oscillations and resulted in a subsequent cessation of spontaneous electrical activity and associated Ca2+ spiking, which persisted after [Ca2+]i returned to baseline. Individual cells in the monolayer responded to arginine vasopressin with a different latency. Agonist-induced Ca2+ waves within one cell propagated much more slowly than spontaneous [Ca2+]i rises. We conclude that agonist-induced [Ca2+]i increases in an electrically coupled cell monolayer can be asynchronous.

Original languageEnglish
Pages (from-to)283-287
Number of pages5
JournalPflügers Archiv European Journal of Physiology
Volume428
Issue number3-4
DOIs
Publication statusPublished - Oct 1 1994
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Physiology
  • Clinical Biochemistry
  • Physiology (medical)

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  • Cite this

    Missiaen, L., Oike, M., Bootman, M. D., De Smedt, H., Parys, J. B., & Casteels, R. (1994). Vasopressin responses in electrically coupled A7r5 cells. Pflügers Archiv European Journal of Physiology, 428(3-4), 283-287. https://doi.org/10.1007/BF00724508