Vasorelaxant effect of 5′-methylthioadenosine obtained from candida utilis yeast extract through the suppression of intracellular Ca2+ concentration in isolated rat aorta

Thanutchaporn Kumrungsee, Sayaka Akiyama, Tomomi Saiki, Masato Omae, Kazuhiro Hamasawa, Toshiro Matsui

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Our study is the first to demonstrate the vasorelaxant effect of Candida utilis yeast extract on rat aorta (EC50 of 7.2 ± 3.2 mg/mL). Among five identified compounds, 5′-methylthioadenosine (MTA) exhibited comparable vasorelaxant effect (EC50 of 190 ± 40 μM) with adenosine, a known vasodilator, on 1 μM phenylephrine (PE)-contracted Sprague-Dawley rat aortic rings. MTA induced vasorelaxation in an endothelium-independent manner and independent of the adenosine receptors. MTA reduced a CaCl2-induced vasocontraction stimulated by 1 μM PE, whereas the effect was abolished in a 60 mM KCl-induced vasocontraction. This indicates that MTA was not involved in the suppression of extracellular Ca2+ influx. MTA significantly (P < 0.01) attenuated the PE-induced activation of calmodulin-dependent kinase II (CaMK II) in aortic rings and inhibited the phosphorylation of L-type Ca2+ channel (VDCC). In conclusion, the underlying mechanism(s) of MTA-induced vasorelaxation involves the inhibition of Ca2+/CaMK II/VDCC phosphorylation pathway, resulting in the suppression of intracellular Ca2+ concentration in aortic rings.

Original languageEnglish
Pages (from-to)3362-3370
Number of pages9
JournalJournal of Agricultural and Food Chemistry
Volume64
Issue number17
DOIs
Publication statusPublished - May 4 2016

Fingerprint

Candida utilis
vasodilation
Candida
yeast extract
aorta
Vasodilator Agents
Yeast
phenylephrine
Aorta
Rats
Yeasts
calcium
rats
Phenylephrine
adenosine
phosphorylation
Calcium-Calmodulin-Dependent Protein Kinases
Phosphorylation
Calmodulin
Calcium Channels

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

Cite this

Vasorelaxant effect of 5′-methylthioadenosine obtained from candida utilis yeast extract through the suppression of intracellular Ca2+ concentration in isolated rat aorta. / Kumrungsee, Thanutchaporn; Akiyama, Sayaka; Saiki, Tomomi; Omae, Masato; Hamasawa, Kazuhiro; Matsui, Toshiro.

In: Journal of Agricultural and Food Chemistry, Vol. 64, No. 17, 04.05.2016, p. 3362-3370.

Research output: Contribution to journalArticle

@article{f8d2f83f87df47e4bf2bbb4e8d7bba77,
title = "Vasorelaxant effect of 5′-methylthioadenosine obtained from candida utilis yeast extract through the suppression of intracellular Ca2+ concentration in isolated rat aorta",
abstract = "Our study is the first to demonstrate the vasorelaxant effect of Candida utilis yeast extract on rat aorta (EC50 of 7.2 ± 3.2 mg/mL). Among five identified compounds, 5′-methylthioadenosine (MTA) exhibited comparable vasorelaxant effect (EC50 of 190 ± 40 μM) with adenosine, a known vasodilator, on 1 μM phenylephrine (PE)-contracted Sprague-Dawley rat aortic rings. MTA induced vasorelaxation in an endothelium-independent manner and independent of the adenosine receptors. MTA reduced a CaCl2-induced vasocontraction stimulated by 1 μM PE, whereas the effect was abolished in a 60 mM KCl-induced vasocontraction. This indicates that MTA was not involved in the suppression of extracellular Ca2+ influx. MTA significantly (P < 0.01) attenuated the PE-induced activation of calmodulin-dependent kinase II (CaMK II) in aortic rings and inhibited the phosphorylation of L-type Ca2+ channel (VDCC). In conclusion, the underlying mechanism(s) of MTA-induced vasorelaxation involves the inhibition of Ca2+/CaMK II/VDCC phosphorylation pathway, resulting in the suppression of intracellular Ca2+ concentration in aortic rings.",
author = "Thanutchaporn Kumrungsee and Sayaka Akiyama and Tomomi Saiki and Masato Omae and Kazuhiro Hamasawa and Toshiro Matsui",
year = "2016",
month = "5",
day = "4",
doi = "10.1021/acs.jafc.6b00679",
language = "English",
volume = "64",
pages = "3362--3370",
journal = "Journal of Agricultural and Food Chemistry",
issn = "0021-8561",
publisher = "American Chemical Society",
number = "17",

}

TY - JOUR

T1 - Vasorelaxant effect of 5′-methylthioadenosine obtained from candida utilis yeast extract through the suppression of intracellular Ca2+ concentration in isolated rat aorta

AU - Kumrungsee, Thanutchaporn

AU - Akiyama, Sayaka

AU - Saiki, Tomomi

AU - Omae, Masato

AU - Hamasawa, Kazuhiro

AU - Matsui, Toshiro

PY - 2016/5/4

Y1 - 2016/5/4

N2 - Our study is the first to demonstrate the vasorelaxant effect of Candida utilis yeast extract on rat aorta (EC50 of 7.2 ± 3.2 mg/mL). Among five identified compounds, 5′-methylthioadenosine (MTA) exhibited comparable vasorelaxant effect (EC50 of 190 ± 40 μM) with adenosine, a known vasodilator, on 1 μM phenylephrine (PE)-contracted Sprague-Dawley rat aortic rings. MTA induced vasorelaxation in an endothelium-independent manner and independent of the adenosine receptors. MTA reduced a CaCl2-induced vasocontraction stimulated by 1 μM PE, whereas the effect was abolished in a 60 mM KCl-induced vasocontraction. This indicates that MTA was not involved in the suppression of extracellular Ca2+ influx. MTA significantly (P < 0.01) attenuated the PE-induced activation of calmodulin-dependent kinase II (CaMK II) in aortic rings and inhibited the phosphorylation of L-type Ca2+ channel (VDCC). In conclusion, the underlying mechanism(s) of MTA-induced vasorelaxation involves the inhibition of Ca2+/CaMK II/VDCC phosphorylation pathway, resulting in the suppression of intracellular Ca2+ concentration in aortic rings.

AB - Our study is the first to demonstrate the vasorelaxant effect of Candida utilis yeast extract on rat aorta (EC50 of 7.2 ± 3.2 mg/mL). Among five identified compounds, 5′-methylthioadenosine (MTA) exhibited comparable vasorelaxant effect (EC50 of 190 ± 40 μM) with adenosine, a known vasodilator, on 1 μM phenylephrine (PE)-contracted Sprague-Dawley rat aortic rings. MTA induced vasorelaxation in an endothelium-independent manner and independent of the adenosine receptors. MTA reduced a CaCl2-induced vasocontraction stimulated by 1 μM PE, whereas the effect was abolished in a 60 mM KCl-induced vasocontraction. This indicates that MTA was not involved in the suppression of extracellular Ca2+ influx. MTA significantly (P < 0.01) attenuated the PE-induced activation of calmodulin-dependent kinase II (CaMK II) in aortic rings and inhibited the phosphorylation of L-type Ca2+ channel (VDCC). In conclusion, the underlying mechanism(s) of MTA-induced vasorelaxation involves the inhibition of Ca2+/CaMK II/VDCC phosphorylation pathway, resulting in the suppression of intracellular Ca2+ concentration in aortic rings.

UR - http://www.scopus.com/inward/record.url?scp=84968817553&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84968817553&partnerID=8YFLogxK

U2 - 10.1021/acs.jafc.6b00679

DO - 10.1021/acs.jafc.6b00679

M3 - Article

C2 - 27066696

AN - SCOPUS:84968817553

VL - 64

SP - 3362

EP - 3370

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

SN - 0021-8561

IS - 17

ER -