TY - JOUR
T1 - Vba2p, A vacuolar membrane protein involved in basic amino acid transport in schizosaccharomyces pombe
AU - Sugimoto, Naoko
AU - Iwaki, Tomoko
AU - Chardwiriyapreecha, Soracom
AU - Shimazu, Masamitsu
AU - Sekito, Takayuki
AU - Takegawa, Kaoru
AU - Kakinuma, Yoshimi
N1 - Funding Information:
We thank Ms. Mai Nakase for technical assistance, and Dr. Taro Nakamura for providing S. pombe plasmid. Part of this work was performed at the Venture Business Laboratory of Ehime University and it was supported in part by a Grant-in-Aid for Scientific Research (to Y.K.) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.
PY - 2010
Y1 - 2010
N2 - A recent study filling the gap in the genome sequence in the left arm of chromosome 2 of Schizosaccharomyces pombe revealed a homolog of budding yeast Vba2p, a vacuolar transporter of basic amino acids. GFP-tagged Vba2p in fission yeast was localized to the vacuolar membrane. Upon disruption of vba2, the uptake of several amino acids, including lysine, histidine, and arginine, was impaired. A transient increase in lysine uptake under nitrogen starvation was lowered by this mutation. These findings suggest that Vba2p is involved in basic amino acid transport in S. pombe under diverse conditions.
AB - A recent study filling the gap in the genome sequence in the left arm of chromosome 2 of Schizosaccharomyces pombe revealed a homolog of budding yeast Vba2p, a vacuolar transporter of basic amino acids. GFP-tagged Vba2p in fission yeast was localized to the vacuolar membrane. Upon disruption of vba2, the uptake of several amino acids, including lysine, histidine, and arginine, was impaired. A transient increase in lysine uptake under nitrogen starvation was lowered by this mutation. These findings suggest that Vba2p is involved in basic amino acid transport in S. pombe under diverse conditions.
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U2 - 10.1271/bbb.100627
DO - 10.1271/bbb.100627
M3 - Article
C2 - 20944394
AN - SCOPUS:78049368896
SN - 0916-8451
VL - 74
SP - 2166
EP - 2169
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
IS - 10
ER -