Verapamil Prevents Impairment in Filterability of Human Erythrocytes Exposed to Oxidative Stress

Kazuhiko Okamoto, Toru Maruyama, Yoshikazu Kaji, Mine Harada, Shiro Mawatari, Takehiko Fujino, Nobuhiro Uyesaka

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Effects of oxidative stress on intact human erythrocytes were investigated using tert-butyl hydroperoxide (tBHP). Exposure of erythrocytes to tBHP caused a marked decrease in filterability in a time-dependent manner. Erythrocytes exposed to tBHP also show an increase in mean corpuscular volume and a remarkable formation of methemoglobin (met-Hb) without any appearance of hemichromes that form Heinz bodies. High performance liquid chromatography demonstrated that the tBHP-treated erythrocytes exhibited an apparent decrease in the membrane phospholipid, phosphatidylethanolamine (PE). The decrease in PE was inhibited by pretreatment with ascorbate, but not with verapamil. SDS-polyacrylamide gel electrophoresis of the tBHP-treated erythrocyte membrane showed a degradation of spectrin, band 3, band 4.2, and band 4.5, accompanied by the appearance of low-molecular-weight products. The degradation of the membrane proteins was not prevented by pretreatment with verapamil or ascorbate. However, the pretreatment with verapamil but not with ascorbate revealed significant inhibition of the tBHP-induced impairment in filterability in the presence of extracellular Ca2+. Thus, the present study shows that verapamil, a potent drug in reperfusion therapy, plays an important role in protection against oxidative injury, based on a close linkage among decreased filterability, met-Hb formation, and impaired membrane integrity.

Original languageEnglish
Pages (from-to)39-46
Number of pages8
JournalJapanese Journal of Physiology
Volume54
Issue number1
DOIs
Publication statusPublished - Feb 1 2004

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Erythrocyte Deformability
tert-Butylhydroperoxide
Verapamil
Oxidative Stress
Erythrocytes
Methemoglobin
Heinz Bodies
Play Therapy
Spectrin
Erythrocyte Indices
Membranes
Erythrocyte Membrane
Reperfusion
Polyacrylamide Gel Electrophoresis
Phospholipids
Membrane Proteins
Molecular Weight
High Pressure Liquid Chromatography
Wounds and Injuries
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Physiology

Cite this

Verapamil Prevents Impairment in Filterability of Human Erythrocytes Exposed to Oxidative Stress. / Okamoto, Kazuhiko; Maruyama, Toru; Kaji, Yoshikazu; Harada, Mine; Mawatari, Shiro; Fujino, Takehiko; Uyesaka, Nobuhiro.

In: Japanese Journal of Physiology, Vol. 54, No. 1, 01.02.2004, p. 39-46.

Research output: Contribution to journalArticle

Okamoto, Kazuhiko ; Maruyama, Toru ; Kaji, Yoshikazu ; Harada, Mine ; Mawatari, Shiro ; Fujino, Takehiko ; Uyesaka, Nobuhiro. / Verapamil Prevents Impairment in Filterability of Human Erythrocytes Exposed to Oxidative Stress. In: Japanese Journal of Physiology. 2004 ; Vol. 54, No. 1. pp. 39-46.
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