Versatile glycoblotting nanoparticles for high-throughput protein glycomics

Kenichi Niikura, Ryosuke Kamitani, Masaki Kurogochi, Rie Uematsu, Yasuro Shinohara, Hiroaki Nakagawa, Kisaburo Deguchi, Kenji Monde, Hirosato Kondo, Shin Ichiro Nishimura

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32 Citations (Scopus)

Abstract

We have developed an effective and practical trap-and-release method based on chemoselective ligation of carbohydrates with reactive aminooxyl groups attached to the surface of nanoparticles (referred to as glycoblotting nanoparticles). These glycoblotting nanoparticles were synthesized by UV irradiation of diacetylene-functionalized lipids that contain the aminooxyl group. The glycoblotting nanoparticles captured carbohydrates in aqueous solution under mild conditions and were collected by simple centrifugation. The trapped carbohydrates were effectively released from the nanoparticles under acidic conditions to give pure oligosaccharides. This glycoblotting process reduced the time required for the purification process of carbohydrates to less than 6 h, compared to the several days needed for conventional chromatographic techniques. The oligosaccharides (N-glycan) were released from ovalbumin (glycoprotein) by PNGase F after tryptic digestion. MALDI-TOF mass spectra before purification did not show any significant signals corresponding to N-glycans because these signals were hidden by the large signals of the abundant peplides. However, after purification with the glycoblotting nanoparticles, only signals corresponding to oligosaccharides appeared. We also demonstrated a clear analysis of the oligosaccharides contained in the mice dermis by means of glycoblotting.

Original languageEnglish
Pages (from-to)3825-3834
Number of pages10
JournalChemistry - A European Journal
Volume11
Issue number13
DOIs
Publication statusPublished - Jun 20 2005
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Catalysis
  • Organic Chemistry

Cite this

Niikura, K., Kamitani, R., Kurogochi, M., Uematsu, R., Shinohara, Y., Nakagawa, H., ... Nishimura, S. I. (2005). Versatile glycoblotting nanoparticles for high-throughput protein glycomics. Chemistry - A European Journal, 11(13), 3825-3834. https://doi.org/10.1002/chem.200401289