Viability loss and oxidation during desiccation of recalcitrant Pachira macrocarpa Seeds

Rong Show Shen, Shao Wei Lu, Kuang Liang Huang, Yu Sen Chang, Ikuo Miyajima

Research output: Contribution to journalArticle

Abstract

This study investigated the correlation between the moisture content and viability of Pachira macrocarpa seeds, and observed the changes in seed oxidation and oxidation resistance during the desiccation process. The average moisture content of a fresh mature P. macrocarpa seed was approximately 1.93 gH2O·g-1 DW. When the seeds were placed into desiccating environments at 25°C with 60% relative humidity, seed viability and moisture content exhibited highly curve correlation. The R2 reached 0.98, and seed viability rapidly declined as the moisture content decreased. This test was observed causing seed death occurred at a critical moisture content of 1.4gH2O·g-1 DW, where the seed germination percentage was 0%. This indicates that P. macrocarpa seeds are not only recalcitrant but also sensitive to desiccation. Furthermore, the seed oxidation resistance showed that after 0-5 days of desiccation, the superoxide anion radical (O2-) and hydrogen peroxide (H2O2) levels increased as the seed moisture content decreased. The activity of the antioxidant enzyme superoxidase dismutase (SOD) and ascorbate peroxidase (APX) also decreased with the seed moisture content. When the seeds were desiccated to a moisture content of 1.22 gH2O·g-1 DW, the SOD activity was reduced to half that of a fresh seed. Although the ascorbate-glutathione cycle continued to function, the seed's malondialdehyde (MDA) content increased 1.5 times, which expedited seed oxidation. However, the reduction of P. macrocarpa seed viability during desiccation occurred before significant oxidation, while seed death may have been caused by physical damage not metabolic injury.

Original languageEnglish
Pages (from-to)23-32
Number of pages10
JournalJournal of the Faculty of Agriculture, Kyushu University
Volume60
Issue number1
Publication statusPublished - Jan 1 2015
Externally publishedYes

Fingerprint

Pachira aquatica
Bombacaceae
Desiccation
desiccation (plant physiology)
Seeds
viability
oxidation
seeds
water content
Superoxides

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science

Cite this

Viability loss and oxidation during desiccation of recalcitrant Pachira macrocarpa Seeds. / Shen, Rong Show; Lu, Shao Wei; Huang, Kuang Liang; Chang, Yu Sen; Miyajima, Ikuo.

In: Journal of the Faculty of Agriculture, Kyushu University, Vol. 60, No. 1, 01.01.2015, p. 23-32.

Research output: Contribution to journalArticle

Shen, Rong Show ; Lu, Shao Wei ; Huang, Kuang Liang ; Chang, Yu Sen ; Miyajima, Ikuo. / Viability loss and oxidation during desiccation of recalcitrant Pachira macrocarpa Seeds. In: Journal of the Faculty of Agriculture, Kyushu University. 2015 ; Vol. 60, No. 1. pp. 23-32.
@article{9a5609ced2db416bb56380ba8ea70e10,
title = "Viability loss and oxidation during desiccation of recalcitrant Pachira macrocarpa Seeds",
abstract = "This study investigated the correlation between the moisture content and viability of Pachira macrocarpa seeds, and observed the changes in seed oxidation and oxidation resistance during the desiccation process. The average moisture content of a fresh mature P. macrocarpa seed was approximately 1.93 gH2O·g-1 DW. When the seeds were placed into desiccating environments at 25°C with 60{\%} relative humidity, seed viability and moisture content exhibited highly curve correlation. The R2 reached 0.98, and seed viability rapidly declined as the moisture content decreased. This test was observed causing seed death occurred at a critical moisture content of 1.4gH2O·g-1 DW, where the seed germination percentage was 0{\%}. This indicates that P. macrocarpa seeds are not only recalcitrant but also sensitive to desiccation. Furthermore, the seed oxidation resistance showed that after 0-5 days of desiccation, the superoxide anion radical (O2-) and hydrogen peroxide (H2O2) levels increased as the seed moisture content decreased. The activity of the antioxidant enzyme superoxidase dismutase (SOD) and ascorbate peroxidase (APX) also decreased with the seed moisture content. When the seeds were desiccated to a moisture content of 1.22 gH2O·g-1 DW, the SOD activity was reduced to half that of a fresh seed. Although the ascorbate-glutathione cycle continued to function, the seed's malondialdehyde (MDA) content increased 1.5 times, which expedited seed oxidation. However, the reduction of P. macrocarpa seed viability during desiccation occurred before significant oxidation, while seed death may have been caused by physical damage not metabolic injury.",
author = "Shen, {Rong Show} and Lu, {Shao Wei} and Huang, {Kuang Liang} and Chang, {Yu Sen} and Ikuo Miyajima",
year = "2015",
month = "1",
day = "1",
language = "English",
volume = "60",
pages = "23--32",
journal = "Journal of the Faculty of Agriculture, Kyushu University",
issn = "0023-6152",
publisher = "Faculty of Agriculture, Kyushu University",
number = "1",

}

TY - JOUR

T1 - Viability loss and oxidation during desiccation of recalcitrant Pachira macrocarpa Seeds

AU - Shen, Rong Show

AU - Lu, Shao Wei

AU - Huang, Kuang Liang

AU - Chang, Yu Sen

AU - Miyajima, Ikuo

PY - 2015/1/1

Y1 - 2015/1/1

N2 - This study investigated the correlation between the moisture content and viability of Pachira macrocarpa seeds, and observed the changes in seed oxidation and oxidation resistance during the desiccation process. The average moisture content of a fresh mature P. macrocarpa seed was approximately 1.93 gH2O·g-1 DW. When the seeds were placed into desiccating environments at 25°C with 60% relative humidity, seed viability and moisture content exhibited highly curve correlation. The R2 reached 0.98, and seed viability rapidly declined as the moisture content decreased. This test was observed causing seed death occurred at a critical moisture content of 1.4gH2O·g-1 DW, where the seed germination percentage was 0%. This indicates that P. macrocarpa seeds are not only recalcitrant but also sensitive to desiccation. Furthermore, the seed oxidation resistance showed that after 0-5 days of desiccation, the superoxide anion radical (O2-) and hydrogen peroxide (H2O2) levels increased as the seed moisture content decreased. The activity of the antioxidant enzyme superoxidase dismutase (SOD) and ascorbate peroxidase (APX) also decreased with the seed moisture content. When the seeds were desiccated to a moisture content of 1.22 gH2O·g-1 DW, the SOD activity was reduced to half that of a fresh seed. Although the ascorbate-glutathione cycle continued to function, the seed's malondialdehyde (MDA) content increased 1.5 times, which expedited seed oxidation. However, the reduction of P. macrocarpa seed viability during desiccation occurred before significant oxidation, while seed death may have been caused by physical damage not metabolic injury.

AB - This study investigated the correlation between the moisture content and viability of Pachira macrocarpa seeds, and observed the changes in seed oxidation and oxidation resistance during the desiccation process. The average moisture content of a fresh mature P. macrocarpa seed was approximately 1.93 gH2O·g-1 DW. When the seeds were placed into desiccating environments at 25°C with 60% relative humidity, seed viability and moisture content exhibited highly curve correlation. The R2 reached 0.98, and seed viability rapidly declined as the moisture content decreased. This test was observed causing seed death occurred at a critical moisture content of 1.4gH2O·g-1 DW, where the seed germination percentage was 0%. This indicates that P. macrocarpa seeds are not only recalcitrant but also sensitive to desiccation. Furthermore, the seed oxidation resistance showed that after 0-5 days of desiccation, the superoxide anion radical (O2-) and hydrogen peroxide (H2O2) levels increased as the seed moisture content decreased. The activity of the antioxidant enzyme superoxidase dismutase (SOD) and ascorbate peroxidase (APX) also decreased with the seed moisture content. When the seeds were desiccated to a moisture content of 1.22 gH2O·g-1 DW, the SOD activity was reduced to half that of a fresh seed. Although the ascorbate-glutathione cycle continued to function, the seed's malondialdehyde (MDA) content increased 1.5 times, which expedited seed oxidation. However, the reduction of P. macrocarpa seed viability during desiccation occurred before significant oxidation, while seed death may have been caused by physical damage not metabolic injury.

UR - http://www.scopus.com/inward/record.url?scp=84927768580&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84927768580&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:84927768580

VL - 60

SP - 23

EP - 32

JO - Journal of the Faculty of Agriculture, Kyushu University

JF - Journal of the Faculty of Agriculture, Kyushu University

SN - 0023-6152

IS - 1

ER -