TY - JOUR
T1 - Viability of deformed cells
AU - Takamatsu, Hiroshi
AU - Rubinsky, Boris
N1 - Funding Information:
Received April 26, 1999; accepted September 23, 1999. This work was supported by the Program for Overseas Researchers from the Ministry of Education, Japan.
PY - 1999/11
Y1 - 1999/11
N2 - Most of the researchers in the field of cryobiology believe that the mechanism of damage during freezing with low cooling rates is chemical and related to the hypertonicity of the extracellular solution. However, there is some evidence to indicate that cells may be destroyed during freezing also by compression between ice crystals. We have developed an experimental procedure to study the effect of cell compression on viability. Using human prostate primary adenoma cancer cells we show that cell viability decreases steeply when cells are compressed to 30% of their original diameter. If uniform expansion of cell membrane is assumed, this corresponds to a 50% increase in the cell membrane surface area. A simple mathematical model shows that the temperature at which the compression effect may cause cell damage is related to the spacing between ice crystals. When the ice crystals are spaced at distances comparable to the cell diameter the model combined with our experimental data predicts compression damage at about - 1.8°C. This is consistent with experimental observation on frozen cell destruction in the presence of antifreeze proteins.
AB - Most of the researchers in the field of cryobiology believe that the mechanism of damage during freezing with low cooling rates is chemical and related to the hypertonicity of the extracellular solution. However, there is some evidence to indicate that cells may be destroyed during freezing also by compression between ice crystals. We have developed an experimental procedure to study the effect of cell compression on viability. Using human prostate primary adenoma cancer cells we show that cell viability decreases steeply when cells are compressed to 30% of their original diameter. If uniform expansion of cell membrane is assumed, this corresponds to a 50% increase in the cell membrane surface area. A simple mathematical model shows that the temperature at which the compression effect may cause cell damage is related to the spacing between ice crystals. When the ice crystals are spaced at distances comparable to the cell diameter the model combined with our experimental data predicts compression damage at about - 1.8°C. This is consistent with experimental observation on frozen cell destruction in the presence of antifreeze proteins.
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U2 - 10.1006/cryo.1999.2207
DO - 10.1006/cryo.1999.2207
M3 - Article
C2 - 10600258
AN - SCOPUS:0033372490
SN - 0011-2240
VL - 39
SP - 243
EP - 251
JO - Cryobiology
JF - Cryobiology
IS - 3
ER -