Vibrio aphrogenes sp. nov., in the Rumoiensis clade isolated from a seaweed

Mami Tanaka, Shoko Endo, Fumihito Kotake, Nurhidayu Al-Saari, A. K.M.Rohul Amin, Gao Feng, Sayaka Mino, Hidetaka Doi, Yoshitoshi Ogura, Tetsuya Hayashi, Wataru Suda, Masahira Hattori, Isao Yumoto, Toko Sawabe, Tomoo Sawabe, Toshiyoshi Araki

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A novel strain Vibrio aphrogenes sp. nov. strain CA-1004T isolated from the surface of seaweed collected on the coast of Mie Prefecture in 1994 [1] was characterized using polyphasic taxonomy including multilocus sequence analysis (MLSA) and a genome based comparison. Both phylogenetic analyses on the basis of 16S rRNA gene sequences and MLSA based on eight protein-coding genes (gapA, gyrB, ftsZ, mreB, pyrH, recA, rpoA, and topA) showed the strain could be placed in the Rumoiensis clade in the genus Vibrio. Sequence similarities of the 16S rRNA gene and the multilocus genes against the Rumoiensis clade members, V. rumoiensis, V. algivorus, V. casei, and V. litoralis, were low enough to propose V. aphrogenes sp. nov. strain CA-1004T as a separate species. The experimental DNA-DNA hybridization data also revealed that the strain CA-1004T was separate from four known Rumoiensis clade species. The G+C content of the V. aphrogenes strain was determined as 42.1% based on the genome sequence. Major traits of the strain were nonmotile, halophilic, fermentative, alginolytic, and gas production. A total of 27 traits (motility, growth temperature range, amylase, alginase and lipase productions, and assimilation of 19 carbon compounds) distinguished the strain from the other species in the Rumoiensis clade. The name V. aphrogenes sp. nov. is proposed for this species in the Rumoiensis clade, with CA-1004T as the type strain (JCM 31643T = DSM 103759T).

Original languageEnglish
Article numbere0180053
JournalPloS one
Volume12
Issue number6
DOIs
Publication statusPublished - Jun 2017

Fingerprint

Seaweed
Multilocus Sequence Typing
Vibrio
rRNA Genes
macroalgae
Genome
new species
DNA
Base Composition
Amylases
Genes
Lipase
Names
Carbon
Gases
Temperature
Growth
Vibrio rumoiensis
Proteins
ribosomal RNA

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Tanaka, M., Endo, S., Kotake, F., Al-Saari, N., Amin, A. K. M. R., Feng, G., ... Araki, T. (2017). Vibrio aphrogenes sp. nov., in the Rumoiensis clade isolated from a seaweed. PloS one, 12(6), [e0180053]. https://doi.org/10.1371/journal.pone.0180053

Vibrio aphrogenes sp. nov., in the Rumoiensis clade isolated from a seaweed. / Tanaka, Mami; Endo, Shoko; Kotake, Fumihito; Al-Saari, Nurhidayu; Amin, A. K.M.Rohul; Feng, Gao; Mino, Sayaka; Doi, Hidetaka; Ogura, Yoshitoshi; Hayashi, Tetsuya; Suda, Wataru; Hattori, Masahira; Yumoto, Isao; Sawabe, Toko; Sawabe, Tomoo; Araki, Toshiyoshi.

In: PloS one, Vol. 12, No. 6, e0180053, 06.2017.

Research output: Contribution to journalArticle

Tanaka, M, Endo, S, Kotake, F, Al-Saari, N, Amin, AKMR, Feng, G, Mino, S, Doi, H, Ogura, Y, Hayashi, T, Suda, W, Hattori, M, Yumoto, I, Sawabe, T, Sawabe, T & Araki, T 2017, 'Vibrio aphrogenes sp. nov., in the Rumoiensis clade isolated from a seaweed', PloS one, vol. 12, no. 6, e0180053. https://doi.org/10.1371/journal.pone.0180053
Tanaka M, Endo S, Kotake F, Al-Saari N, Amin AKMR, Feng G et al. Vibrio aphrogenes sp. nov., in the Rumoiensis clade isolated from a seaweed. PloS one. 2017 Jun;12(6). e0180053. https://doi.org/10.1371/journal.pone.0180053
Tanaka, Mami ; Endo, Shoko ; Kotake, Fumihito ; Al-Saari, Nurhidayu ; Amin, A. K.M.Rohul ; Feng, Gao ; Mino, Sayaka ; Doi, Hidetaka ; Ogura, Yoshitoshi ; Hayashi, Tetsuya ; Suda, Wataru ; Hattori, Masahira ; Yumoto, Isao ; Sawabe, Toko ; Sawabe, Tomoo ; Araki, Toshiyoshi. / Vibrio aphrogenes sp. nov., in the Rumoiensis clade isolated from a seaweed. In: PloS one. 2017 ; Vol. 12, No. 6.
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abstract = "A novel strain Vibrio aphrogenes sp. nov. strain CA-1004T isolated from the surface of seaweed collected on the coast of Mie Prefecture in 1994 [1] was characterized using polyphasic taxonomy including multilocus sequence analysis (MLSA) and a genome based comparison. Both phylogenetic analyses on the basis of 16S rRNA gene sequences and MLSA based on eight protein-coding genes (gapA, gyrB, ftsZ, mreB, pyrH, recA, rpoA, and topA) showed the strain could be placed in the Rumoiensis clade in the genus Vibrio. Sequence similarities of the 16S rRNA gene and the multilocus genes against the Rumoiensis clade members, V. rumoiensis, V. algivorus, V. casei, and V. litoralis, were low enough to propose V. aphrogenes sp. nov. strain CA-1004T as a separate species. The experimental DNA-DNA hybridization data also revealed that the strain CA-1004T was separate from four known Rumoiensis clade species. The G+C content of the V. aphrogenes strain was determined as 42.1{\%} based on the genome sequence. Major traits of the strain were nonmotile, halophilic, fermentative, alginolytic, and gas production. A total of 27 traits (motility, growth temperature range, amylase, alginase and lipase productions, and assimilation of 19 carbon compounds) distinguished the strain from the other species in the Rumoiensis clade. The name V. aphrogenes sp. nov. is proposed for this species in the Rumoiensis clade, with CA-1004T as the type strain (JCM 31643T = DSM 103759T).",
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