Visual detection of saikosaponins by on-membrane immunoassay and estimation of traditional Chinese medicines containing Bupleuri radix

Osamu Morinaga, Shuhang Zhu, Hiroyuki Tanaka, Yukihiro Shoyama

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The purpose of this study was to describe the simple, rapid, and environmental-cost effective determination method for saikosaponins in complicated samples like Bupleuri radix and traditional Chinese medicines (TCM). Saikosaponin standards, extracts of Bupleuri radix and TCM, were applied to a polyethersulphone (PES) membrane and developed by acetonitrile-water (1:4, by volume). Saikosaponin a (SSa), SSc, and SSd were visually detected by an immunostaining method (called Eastern blotting technique) using a monoclonal antibody (MAb) against SSa. At least 62.5 ng of SSa, SSc, and SSd were clearly detectable individually. These coloring spot areas of saikosaponins on PES membrane were calculated by using the NIH Imaging software and three saikosaponins can be analyzed quantitatively between 62.5 ng and 1.0 μg. Saikosaponins in Bupleuri radix and TCM were determined and these results of SSa and total saikosaponin concentrations were in good agreement with those from the ELISA analysis.

Original languageEnglish
Pages (from-to)687-692
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume346
Issue number3
DOIs
Publication statusPublished - Aug 4 2006

Fingerprint

Chinese Traditional Medicine
Immunoassay
Medicine
Membranes
saikosaponin
Coloring
Software
Enzyme-Linked Immunosorbent Assay
Monoclonal Antibodies
Imaging techniques
Costs and Cost Analysis
Water

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Visual detection of saikosaponins by on-membrane immunoassay and estimation of traditional Chinese medicines containing Bupleuri radix. / Morinaga, Osamu; Zhu, Shuhang; Tanaka, Hiroyuki; Shoyama, Yukihiro.

In: Biochemical and Biophysical Research Communications, Vol. 346, No. 3, 04.08.2006, p. 687-692.

Research output: Contribution to journalArticle

@article{29aca49f733e4f5f85ae1e86ec588ce3,
title = "Visual detection of saikosaponins by on-membrane immunoassay and estimation of traditional Chinese medicines containing Bupleuri radix",
abstract = "The purpose of this study was to describe the simple, rapid, and environmental-cost effective determination method for saikosaponins in complicated samples like Bupleuri radix and traditional Chinese medicines (TCM). Saikosaponin standards, extracts of Bupleuri radix and TCM, were applied to a polyethersulphone (PES) membrane and developed by acetonitrile-water (1:4, by volume). Saikosaponin a (SSa), SSc, and SSd were visually detected by an immunostaining method (called Eastern blotting technique) using a monoclonal antibody (MAb) against SSa. At least 62.5 ng of SSa, SSc, and SSd were clearly detectable individually. These coloring spot areas of saikosaponins on PES membrane were calculated by using the NIH Imaging software and three saikosaponins can be analyzed quantitatively between 62.5 ng and 1.0 μg. Saikosaponins in Bupleuri radix and TCM were determined and these results of SSa and total saikosaponin concentrations were in good agreement with those from the ELISA analysis.",
author = "Osamu Morinaga and Shuhang Zhu and Hiroyuki Tanaka and Yukihiro Shoyama",
year = "2006",
month = "8",
day = "4",
doi = "10.1016/j.bbrc.2006.05.129",
language = "English",
volume = "346",
pages = "687--692",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - Visual detection of saikosaponins by on-membrane immunoassay and estimation of traditional Chinese medicines containing Bupleuri radix

AU - Morinaga, Osamu

AU - Zhu, Shuhang

AU - Tanaka, Hiroyuki

AU - Shoyama, Yukihiro

PY - 2006/8/4

Y1 - 2006/8/4

N2 - The purpose of this study was to describe the simple, rapid, and environmental-cost effective determination method for saikosaponins in complicated samples like Bupleuri radix and traditional Chinese medicines (TCM). Saikosaponin standards, extracts of Bupleuri radix and TCM, were applied to a polyethersulphone (PES) membrane and developed by acetonitrile-water (1:4, by volume). Saikosaponin a (SSa), SSc, and SSd were visually detected by an immunostaining method (called Eastern blotting technique) using a monoclonal antibody (MAb) against SSa. At least 62.5 ng of SSa, SSc, and SSd were clearly detectable individually. These coloring spot areas of saikosaponins on PES membrane were calculated by using the NIH Imaging software and three saikosaponins can be analyzed quantitatively between 62.5 ng and 1.0 μg. Saikosaponins in Bupleuri radix and TCM were determined and these results of SSa and total saikosaponin concentrations were in good agreement with those from the ELISA analysis.

AB - The purpose of this study was to describe the simple, rapid, and environmental-cost effective determination method for saikosaponins in complicated samples like Bupleuri radix and traditional Chinese medicines (TCM). Saikosaponin standards, extracts of Bupleuri radix and TCM, were applied to a polyethersulphone (PES) membrane and developed by acetonitrile-water (1:4, by volume). Saikosaponin a (SSa), SSc, and SSd were visually detected by an immunostaining method (called Eastern blotting technique) using a monoclonal antibody (MAb) against SSa. At least 62.5 ng of SSa, SSc, and SSd were clearly detectable individually. These coloring spot areas of saikosaponins on PES membrane were calculated by using the NIH Imaging software and three saikosaponins can be analyzed quantitatively between 62.5 ng and 1.0 μg. Saikosaponins in Bupleuri radix and TCM were determined and these results of SSa and total saikosaponin concentrations were in good agreement with those from the ELISA analysis.

UR - http://www.scopus.com/inward/record.url?scp=33745454431&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33745454431&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2006.05.129

DO - 10.1016/j.bbrc.2006.05.129

M3 - Article

C2 - 16780795

AN - SCOPUS:33745454431

VL - 346

SP - 687

EP - 692

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 3

ER -