TY - JOUR
T1 - Visualization and evaluation of the promoter activities of genes for stress-inducible proteins in response to environmental pollutants
AU - Ohashi, Tomoko
AU - Satoh, Tetsuya
AU - Kuwata, Minoru
AU - Fujimura, Taira
AU - Taketani, Shigeru
PY - 2007/4
Y1 - 2007/4
N2 - We examined the systematic assay of the reporter gene for the assessment of heavy metals and organic chemical pollutants using the reporter plasmids carrying stress-responsive elements fused to the green fluorescence protein (GFP) gene as follows: metallothionein (MTIIA), heme oxigenase-1 (HO-1), quinone reductase (ARE), and c-fos genes. The treatment of COS7 cells in which the c-fos gene promoter-, ARE-, or HO-1 enhancer-fused GFP with a low concentration of NaAsO2 was introduced led to the detection of the fluorescent cells, and an agrichemical paraquat enhanced the fluorescence of ARE or HO-1 enhancer-transfected cells. The cells in which the plasmid carrying the MT-IIA gene promoter (the -765 bp upstream from the transcription initiation site) was introduced highly expressed GFP on treatment with CdCl2, ZnSO 4, or CuCl2. The plasmid carrying seven metal-responsive elements of the MT-IIA gene increased the response of the fluorescence intensity to these heavy metals. These results indicated that the use of the gene promoters and enhancers of the stress-responsive genesfused to GFP contributes to the visualization of pollutantresponsive mammalian cells and can be applied to biomonitoring of environmental pollution.
AB - We examined the systematic assay of the reporter gene for the assessment of heavy metals and organic chemical pollutants using the reporter plasmids carrying stress-responsive elements fused to the green fluorescence protein (GFP) gene as follows: metallothionein (MTIIA), heme oxigenase-1 (HO-1), quinone reductase (ARE), and c-fos genes. The treatment of COS7 cells in which the c-fos gene promoter-, ARE-, or HO-1 enhancer-fused GFP with a low concentration of NaAsO2 was introduced led to the detection of the fluorescent cells, and an agrichemical paraquat enhanced the fluorescence of ARE or HO-1 enhancer-transfected cells. The cells in which the plasmid carrying the MT-IIA gene promoter (the -765 bp upstream from the transcription initiation site) was introduced highly expressed GFP on treatment with CdCl2, ZnSO 4, or CuCl2. The plasmid carrying seven metal-responsive elements of the MT-IIA gene increased the response of the fluorescence intensity to these heavy metals. These results indicated that the use of the gene promoters and enhancers of the stress-responsive genesfused to GFP contributes to the visualization of pollutantresponsive mammalian cells and can be applied to biomonitoring of environmental pollution.
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U2 - 10.1248/yakushi.127.757
DO - 10.1248/yakushi.127.757
M3 - Article
C2 - 17409708
AN - SCOPUS:34047226909
VL - 127
SP - 757
EP - 764
JO - Yakugaku Zasshi
JF - Yakugaku Zasshi
SN - 0031-6903
IS - 4
ER -