Visualization and evaluation of the promoter activities of genes for stress-inducible proteins in response to environmental pollutants

Tomoko Ohashi, Tetsuya Sato, Minoru Kuwata, Taira Fujimura, Shigeru Taketani

    Research output: Contribution to journalArticle

    1 Citation (Scopus)

    Abstract

    We examined the systematic assay of the reporter gene for the assessment of heavy metals and organic chemical pollutants using the reporter plasmids carrying stress-responsive elements fused to the green fluorescence protein (GFP) gene as follows: metallothionein (MTIIA), heme oxigenase-1 (HO-1), quinone reductase (ARE), and c-fos genes. The treatment of COS7 cells in which the c-fos gene promoter-, ARE-, or HO-1 enhancer-fused GFP with a low concentration of NaAsO2 was introduced led to the detection of the fluorescent cells, and an agrichemical paraquat enhanced the fluorescence of ARE or HO-1 enhancer-transfected cells. The cells in which the plasmid carrying the MT-IIA gene promoter (the -765 bp upstream from the transcription initiation site) was introduced highly expressed GFP on treatment with CdCl2, ZnSO 4, or CuCl2. The plasmid carrying seven metal-responsive elements of the MT-IIA gene increased the response of the fluorescence intensity to these heavy metals. These results indicated that the use of the gene promoters and enhancers of the stress-responsive genesfused to GFP contributes to the visualization of pollutantresponsive mammalian cells and can be applied to biomonitoring of environmental pollution.

    Original languageEnglish
    Pages (from-to)757-764
    Number of pages8
    JournalYakugaku Zasshi
    Volume127
    Issue number4
    DOIs
    Publication statusPublished - Apr 1 2007

    Fingerprint

    Environmental Pollutants
    Heat-Shock Proteins
    Fluorescence
    Genes
    fos Genes
    Plasmids
    Heavy Metals
    Proteins
    NAD(P)H Dehydrogenase (Quinone)
    Agrochemicals
    Organic Chemicals
    Cadmium Chloride
    Environmental Pollution
    Paraquat
    Metallothionein
    Environmental Monitoring
    Transcription Initiation Site
    Reporter Genes
    Metals
    heme 1

    All Science Journal Classification (ASJC) codes

    • Pharmacology
    • Pharmaceutical Science

    Cite this

    Visualization and evaluation of the promoter activities of genes for stress-inducible proteins in response to environmental pollutants. / Ohashi, Tomoko; Sato, Tetsuya; Kuwata, Minoru; Fujimura, Taira; Taketani, Shigeru.

    In: Yakugaku Zasshi, Vol. 127, No. 4, 01.04.2007, p. 757-764.

    Research output: Contribution to journalArticle

    Ohashi, Tomoko ; Sato, Tetsuya ; Kuwata, Minoru ; Fujimura, Taira ; Taketani, Shigeru. / Visualization and evaluation of the promoter activities of genes for stress-inducible proteins in response to environmental pollutants. In: Yakugaku Zasshi. 2007 ; Vol. 127, No. 4. pp. 757-764.
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