Abstract
We examined the systematic assay of the reporter gene for the assessment of heavy metals and organic chemical pollutants using the reporter plasmids carrying stress-responsive elements fused to the green fluorescence protein (GFP) gene as follows: metallothionein (MTIIA), heme oxigenase-1 (HO-1), quinone reductase (ARE), and c-fos genes. The treatment of COS7 cells in which the c-fos gene promoter-, ARE-, or HO-1 enhancer-fused GFP with a low concentration of NaAsO2 was introduced led to the detection of the fluorescent cells, and an agrichemical paraquat enhanced the fluorescence of ARE or HO-1 enhancer-transfected cells. The cells in which the plasmid carrying the MT-IIA gene promoter (the -765 bp upstream from the transcription initiation site) was introduced highly expressed GFP on treatment with CdCl2, ZnSO 4, or CuCl2. The plasmid carrying seven metal-responsive elements of the MT-IIA gene increased the response of the fluorescence intensity to these heavy metals. These results indicated that the use of the gene promoters and enhancers of the stress-responsive genesfused to GFP contributes to the visualization of pollutantresponsive mammalian cells and can be applied to biomonitoring of environmental pollution.
| Original language | English |
|---|---|
| Pages (from-to) | 757-764 |
| Number of pages | 8 |
| Journal | Yakugaku Zasshi |
| Volume | 127 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Apr 1 2007 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Pharmacology
- Pharmaceutical Science
Cite this
Visualization and evaluation of the promoter activities of genes for stress-inducible proteins in response to environmental pollutants. / Ohashi, Tomoko; Sato, Tetsuya; Kuwata, Minoru; Fujimura, Taira; Taketani, Shigeru.
In: Yakugaku Zasshi, Vol. 127, No. 4, 01.04.2007, p. 757-764.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Visualization and evaluation of the promoter activities of genes for stress-inducible proteins in response to environmental pollutants
AU - Ohashi, Tomoko
AU - Sato, Tetsuya
AU - Kuwata, Minoru
AU - Fujimura, Taira
AU - Taketani, Shigeru
PY - 2007/4/1
Y1 - 2007/4/1
N2 - We examined the systematic assay of the reporter gene for the assessment of heavy metals and organic chemical pollutants using the reporter plasmids carrying stress-responsive elements fused to the green fluorescence protein (GFP) gene as follows: metallothionein (MTIIA), heme oxigenase-1 (HO-1), quinone reductase (ARE), and c-fos genes. The treatment of COS7 cells in which the c-fos gene promoter-, ARE-, or HO-1 enhancer-fused GFP with a low concentration of NaAsO2 was introduced led to the detection of the fluorescent cells, and an agrichemical paraquat enhanced the fluorescence of ARE or HO-1 enhancer-transfected cells. The cells in which the plasmid carrying the MT-IIA gene promoter (the -765 bp upstream from the transcription initiation site) was introduced highly expressed GFP on treatment with CdCl2, ZnSO 4, or CuCl2. The plasmid carrying seven metal-responsive elements of the MT-IIA gene increased the response of the fluorescence intensity to these heavy metals. These results indicated that the use of the gene promoters and enhancers of the stress-responsive genesfused to GFP contributes to the visualization of pollutantresponsive mammalian cells and can be applied to biomonitoring of environmental pollution.
AB - We examined the systematic assay of the reporter gene for the assessment of heavy metals and organic chemical pollutants using the reporter plasmids carrying stress-responsive elements fused to the green fluorescence protein (GFP) gene as follows: metallothionein (MTIIA), heme oxigenase-1 (HO-1), quinone reductase (ARE), and c-fos genes. The treatment of COS7 cells in which the c-fos gene promoter-, ARE-, or HO-1 enhancer-fused GFP with a low concentration of NaAsO2 was introduced led to the detection of the fluorescent cells, and an agrichemical paraquat enhanced the fluorescence of ARE or HO-1 enhancer-transfected cells. The cells in which the plasmid carrying the MT-IIA gene promoter (the -765 bp upstream from the transcription initiation site) was introduced highly expressed GFP on treatment with CdCl2, ZnSO 4, or CuCl2. The plasmid carrying seven metal-responsive elements of the MT-IIA gene increased the response of the fluorescence intensity to these heavy metals. These results indicated that the use of the gene promoters and enhancers of the stress-responsive genesfused to GFP contributes to the visualization of pollutantresponsive mammalian cells and can be applied to biomonitoring of environmental pollution.
UR - http://www.scopus.com/inward/record.url?scp=34047226909&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34047226909&partnerID=8YFLogxK
U2 - 10.1248/yakushi.127.757
DO - 10.1248/yakushi.127.757
M3 - Article
VL - 127
SP - 757
EP - 764
JO - Yakugaku Zasshi
JF - Yakugaku Zasshi
SN - 0031-6903
IS - 4
ER -