TY - JOUR
T1 - Visualization and exploration of Tcf/Lef function using a highly responsive Wnt/Β-catenin signaling-reporter transgenic zebrafish
AU - Shimizu, Nobuyuki
AU - Kawakami, Koichi
AU - Ishitani, Tohru
N1 - Funding Information:
We thank R. Moon, ZIRC, and NBRP zebrafish for providing TOPdGFP, hsp70:Dkk1-GFP, hsp70:ΔTcf3-GFP, and HuC-Kaede transgenic fish, and the tcf7 mutant, as well as the Laboratory for Technical Support, MIB, Kyushu University, the Research Support Center, Graduate School of Medical Sciences, Kyushu University, and Ishitani lab members for their technical support and helpful discussions. This research was supported by the Astellas Foundation for Research on Metabolic Disorders (T.I.), Research Foundation for Opto-Science and Technology (T.I.), Inamori Foundation (T.I.), Shimadzu Foundation (T.I.), Nakajima Foundation (T.I.), Kurata Memorial Hitachi Science and Technology Foundation (T.I), NIG Collaborative Research (T.I. and K.K.), and Grants-in-Aid for Scientific Research programs in Japan (T.I.). Appendix A
PY - 2012/10/1
Y1 - 2012/10/1
N2 - Evolutionarily conserved Tcf/Lef transcription factors (Lef1, Tcf7, Tcf7l1, and Tcf7l2) mediate gene expression regulated by Wnt/Β-catenin signaling, which has multiple roles in early embryogenesis, organogenesis, adult tissue homeostasis, and tissue regeneration. However, the spatiotemporal dynamics of Tcf/Lef activity during these events remain poorly understood. We generated stable transgenic zebrafish lines carrying a new Wnt/Β-catenin signaling reporter, Tcf/Lef-miniP:dGFP. The reporter revealed the transcriptional activities of four Tcf/Lef members controlled by Wnt/Β-catenin signaling, which were expressed in known Wnt/Β-catenin signaling-active sites during embryogenesis, organ development and growth, and tissue regeneration. We used the transgenic lines to demonstrate the contribution of Tcf/Lef-mediated Wnt/Β-catenin signaling to the development of the anterior lateral line, dorsal and secondary posterior lateral lines, and gill filaments. Thus, these reporter lines are highly useful tools for studying Tcf/Lef-mediated Wnt/Β-catenin signaling-dependent processes.
AB - Evolutionarily conserved Tcf/Lef transcription factors (Lef1, Tcf7, Tcf7l1, and Tcf7l2) mediate gene expression regulated by Wnt/Β-catenin signaling, which has multiple roles in early embryogenesis, organogenesis, adult tissue homeostasis, and tissue regeneration. However, the spatiotemporal dynamics of Tcf/Lef activity during these events remain poorly understood. We generated stable transgenic zebrafish lines carrying a new Wnt/Β-catenin signaling reporter, Tcf/Lef-miniP:dGFP. The reporter revealed the transcriptional activities of four Tcf/Lef members controlled by Wnt/Β-catenin signaling, which were expressed in known Wnt/Β-catenin signaling-active sites during embryogenesis, organ development and growth, and tissue regeneration. We used the transgenic lines to demonstrate the contribution of Tcf/Lef-mediated Wnt/Β-catenin signaling to the development of the anterior lateral line, dorsal and secondary posterior lateral lines, and gill filaments. Thus, these reporter lines are highly useful tools for studying Tcf/Lef-mediated Wnt/Β-catenin signaling-dependent processes.
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U2 - 10.1016/j.ydbio.2012.07.016
DO - 10.1016/j.ydbio.2012.07.016
M3 - Article
C2 - 22842099
AN - SCOPUS:84865620520
SN - 0012-1606
VL - 370
SP - 71
EP - 85
JO - Developmental Biology
JF - Developmental Biology
IS - 1
ER -