金属微粒子シートによる細胞接着ナノ界面の可視化

Translated title of the contribution: Visualization of Cell Attached Nanointerface using Metal Nanoparticle Sheet

増田 志穂美, 玉田 薫

Research output: Contribution to journalArticle

Abstract

In this paper, we report a simple, effective method for the visualization of a cell–attached nanointerface using localized surface plasmon resonance (LSPR) excited on a self–assembled metal nanoparticle sheet. The LSPR on the nanoparticle sheet provides high–contrast interfacial images due to the confined light at the nanointerface. The experiment of immobilized RBL–2H3 cells with fluorescence–labeled actin filaments revealed high axial resolution for the focal adhesion even under a regular epifluorescence microscope. This imaging technique can be applied even for live–cell imaging under a total internal reflection fluorescence (TIRF) microscope. This non–scanning–type, high–resolution imaging method will be an effective tool for monitoring interfacial phenomena including molecular level of rapid reaction or various cellular and molecular dynamics.
Translated title of the contributionVisualization of Cell Attached Nanointerface using Metal Nanoparticle Sheet
Original languageJapanese
Pages (from-to)115-120
Number of pages6
JournalMembrane
Volume45
Issue number3
DOIs
Publication statusPublished - 2020

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