Visualization of intracellular lipid metabolism in brown adipocytes by time-lapse ultra-multiplex CARS microspectroscopy with an onstage incubator

Yuki Takei, Rie Hirai, Aya Fukuda, Shinichi Miyazaki, Rintaro Shimada, Yuko Okamatsu-Ogura, Masayuki Saito, Philippe Leproux, Koji Hisatake, Hideaki Kano

Research output: Contribution to journalArticlepeer-review

Abstract

We visualized a dynamic process of fatty acid uptake of brown adipocytes using a time-lapse ultra-broadband multiplex coherent anti-Stokes Raman scattering (CARS) spectroscopic imaging system with an onstage incubator. Combined with the deuterium labeling technique, the intracellular uptake of saturated fatty acids was traced up to 9 h, a substantial advance over the initial multiplex CARS system, with an analysis time of 80 min. Characteristic metabolic activities of brown adipocytes, such as resistance to lipid saturation, were elucidated, supporting the utility of the newly developed system.

Original languageEnglish
Article number125102
JournalJournal of Chemical Physics
Volume155
Issue number12
DOIs
Publication statusPublished - Sep 28 2021

All Science Journal Classification (ASJC) codes

  • Physics and Astronomy(all)
  • Physical and Theoretical Chemistry

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