Visualization of the endocytic pathway in the filamentous fungus Aspergillus oryzae using an EGFP-fused plasma membrane protein

Yujiro Higuchi, Tomoyuki Nakahama, Jun Ya Shoji, Manabu Arioka, Katsuhiko Kitamoto

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Endocytosis is an important process for cellular activities. However, in filamentous fungi, the existence of endocytosis has been so far elusive. In this study, we used AoUapC-EGFP, the fusion protein of a putative uric acid-xanthine permease with enhanced green fluorescent protein (EGFP) in Aspergillus oryzae, to examine whether the endocytic process occurs or not. Upon the addition of ammonium into the medium the fusion protein was internalized from the plasma membrane. The internalization of AoUapC-EGFP was completely blocked by sodium azide, cold, and cytochalasin A treatments, suggesting that the internalization possesses the general features of endocytosis. These results demonstrate the occurrence of endocytosis in filamentous fungi. Moreover, we discovered that the endosomal compartments appeared upon the induction of endocytosis and moved in a microtubule-dependent manner.

Original languageEnglish
Pages (from-to)784-791
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume340
Issue number3
DOIs
Publication statusPublished - Feb 17 2006
Externally publishedYes

Fingerprint

Aspergillus oryzae
Aspergillus
Cell membranes
Endocytosis
Fungi
Blood Proteins
Membrane Proteins
Visualization
Cell Membrane
Fusion reactions
Sodium Azide
Xanthine
Membrane Transport Proteins
Uric Acid
Ammonium Compounds
Proteins
Microtubules
enhanced green fluorescent protein

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Visualization of the endocytic pathway in the filamentous fungus Aspergillus oryzae using an EGFP-fused plasma membrane protein. / Higuchi, Yujiro; Nakahama, Tomoyuki; Shoji, Jun Ya; Arioka, Manabu; Kitamoto, Katsuhiko.

In: Biochemical and Biophysical Research Communications, Vol. 340, No. 3, 17.02.2006, p. 784-791.

Research output: Contribution to journalArticle

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