Visualizing intra-medulla lipids in human hair using ultra-multiplex CARS, SHG, and THG microscopy

Daiki Kaneta, Makiko Goto, Motofumi Hagihara, Philippe Leproux, Vincent Couderc, Mariko Egawa, Hideaki Kano

Research output: Contribution to journalArticlepeer-review

Abstract

We performed label-free imaging of human-hair medulla using multi-modal nonlinear optical microscopy. Intra-medulla lipids (IMLs) were clearly visualized by ultra-multiplex coherent anti-Stokes Raman scattering (CARS) spectroscopic imaging. Two groups of IMLs were found: second harmonic generation (SHG) active and inactive. By combining SHG analysis with CARS, the two groups were identified as free fatty acids and wax esters, respectively.

Original languageEnglish
Pages (from-to)1163-1168
Number of pages6
JournalAnalyst
Volume146
Issue number4
DOIs
Publication statusPublished - Feb 21 2021

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

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