Visualizing intra-medulla lipids in human hair using ultra-multiplex CARS, SHG, and THG microscopy

Daiki Kaneta; Makiko Goto; Motofumi Hagihara; Philippe Leproux; Vincent Couderc; Mariko Egawa; Hideaki Kano

doi:10.1039/d0an01880e

Visualizing intra-medulla lipids in human hair using ultra-multiplex CARS, SHG, and THG microscopy

Daiki Kaneta, Makiko Goto, Motofumi Hagihara, Philippe Leproux, Vincent Couderc, Mariko Egawa, Hideaki Kano

Physical Chemistry

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

We performed label-free imaging of human-hair medulla using multi-modal nonlinear optical microscopy. Intra-medulla lipids (IMLs) were clearly visualized by ultra-multiplex coherent anti-Stokes Raman scattering (CARS) spectroscopic imaging. Two groups of IMLs were found: second harmonic generation (SHG) active and inactive. By combining SHG analysis with CARS, the two groups were identified as free fatty acids and wax esters, respectively.

Original language	English
Pages (from-to)	1163-1168
Number of pages	6
Journal	Analyst
Volume	146
Issue number	4
DOIs	https://doi.org/10.1039/d0an01880e
Publication status	Published - Feb 21 2021

All Science Journal Classification (ASJC) codes

Analytical Chemistry
Biochemistry
Environmental Chemistry
Spectroscopy
Electrochemistry

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10.1039/d0an01880e

Cite this

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title = "Visualizing intra-medulla lipids in human hair using ultra-multiplex CARS, SHG, and THG microscopy",

abstract = "We performed label-free imaging of human-hair medulla using multi-modal nonlinear optical microscopy. Intra-medulla lipids (IMLs) were clearly visualized by ultra-multiplex coherent anti-Stokes Raman scattering (CARS) spectroscopic imaging. Two groups of IMLs were found: second harmonic generation (SHG) active and inactive. By combining SHG analysis with CARS, the two groups were identified as free fatty acids and wax esters, respectively.",

author = "Daiki Kaneta and Makiko Goto and Motofumi Hagihara and Philippe Leproux and Vincent Couderc and Mariko Egawa and Hideaki Kano",

note = "Funding Information: The authors gratefully acknowledge the assistance of J. Ukon of Ukon Craft Science, Ltd, for establishing a fruitful collaboration between Japanese and French laboratories. This study was financially supported by JSPS KAKENHI Grant Number 18H02000 (Grant-in-Aid for Scientific Research [B]). Publisher Copyright: {\textcopyright} The Royal Society of Chemistry.",

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doi = "10.1039/d0an01880e",

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T1 - Visualizing intra-medulla lipids in human hair using ultra-multiplex CARS, SHG, and THG microscopy

AU - Kaneta, Daiki

AU - Goto, Makiko

AU - Hagihara, Motofumi

AU - Leproux, Philippe

AU - Couderc, Vincent

AU - Egawa, Mariko

AU - Kano, Hideaki

N1 - Funding Information: The authors gratefully acknowledge the assistance of J. Ukon of Ukon Craft Science, Ltd, for establishing a fruitful collaboration between Japanese and French laboratories. This study was financially supported by JSPS KAKENHI Grant Number 18H02000 (Grant-in-Aid for Scientific Research [B]). Publisher Copyright: © The Royal Society of Chemistry.

PY - 2021/2/21

Y1 - 2021/2/21

N2 - We performed label-free imaging of human-hair medulla using multi-modal nonlinear optical microscopy. Intra-medulla lipids (IMLs) were clearly visualized by ultra-multiplex coherent anti-Stokes Raman scattering (CARS) spectroscopic imaging. Two groups of IMLs were found: second harmonic generation (SHG) active and inactive. By combining SHG analysis with CARS, the two groups were identified as free fatty acids and wax esters, respectively.

AB - We performed label-free imaging of human-hair medulla using multi-modal nonlinear optical microscopy. Intra-medulla lipids (IMLs) were clearly visualized by ultra-multiplex coherent anti-Stokes Raman scattering (CARS) spectroscopic imaging. Two groups of IMLs were found: second harmonic generation (SHG) active and inactive. By combining SHG analysis with CARS, the two groups were identified as free fatty acids and wax esters, respectively.

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Visualizing intra-medulla lipids in human hair using ultra-multiplex CARS, SHG, and THG microscopy

Abstract

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