Vitamin E Enhances Prostacyclin Production by Cultured Aortic Endothelial Cells

Fumio Umeda, Makoto Kunisaki, Toyoshi Inoguchi, Hajime Nawata

    Research output: Contribution to journalArticle

    5 Citations (Scopus)

    Abstract

    The effect of vitamin E on prostacyclin (PGI2) production by cultured bovine aortic endothelial cells was evaluated in vitro. We found that human plasma-derived serum (PDS) conveys a stimulatory effect on PGI2 production by cultured vascular endothelial cells. In the present study, therefore, PDS was used as a physiological stimulant for PGI2 production. There was no effect of vitamin E alone on PGI2 production without stimulation by PDS. However, simultaneous addition of vitamin E and PDS to the cultures enhanced PGI2 production in a dose-dependent manner. Furthermore, preincubation with vitamin E prior to stimulation with PDS also enhanced PGI2 production. The enhancing effect of preincubation with vitamin E was demonstrated to occur in a time- and dose-dependent manner. These results clearly indicate that vitamin E at physiological doses can enhance PGI2 production by cultured bovine aortic endothelial cells stimulated with an agent such as PDS. It seems likely that vitamin E may contribute to increase PGI2 synthesis by the vascular wall in vivo, thereby reducing platelet aggregation and thrombus formation.

    Original languageEnglish
    Pages (from-to)175-183
    Number of pages9
    JournalJournal of Clinical Biochemistry and Nutrition
    Volume8
    Issue number3
    DOIs
    Publication statusPublished - Jan 1 1990

    Fingerprint

    Endothelial cells
    Epoprostenol
    Vitamin E
    Endothelial Cells
    Plasmas
    Serum
    Plasma (human)
    Platelets
    Platelet Aggregation
    Blood Vessels
    Thrombosis
    Agglomeration

    All Science Journal Classification (ASJC) codes

    • Medicine (miscellaneous)
    • Nutrition and Dietetics
    • Clinical Biochemistry

    Cite this

    Vitamin E Enhances Prostacyclin Production by Cultured Aortic Endothelial Cells. / Umeda, Fumio; Kunisaki, Makoto; Inoguchi, Toyoshi; Nawata, Hajime.

    In: Journal of Clinical Biochemistry and Nutrition, Vol. 8, No. 3, 01.01.1990, p. 175-183.

    Research output: Contribution to journalArticle

    Umeda, Fumio ; Kunisaki, Makoto ; Inoguchi, Toyoshi ; Nawata, Hajime. / Vitamin E Enhances Prostacyclin Production by Cultured Aortic Endothelial Cells. In: Journal of Clinical Biochemistry and Nutrition. 1990 ; Vol. 8, No. 3. pp. 175-183.
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