Vitamin E restores reduced prostacyclin synthesis in aortic endothelial cells cultured with a high concentration of glucose

Makoto Kunisaki, Fumio Umeda, Toyoshi Inoguchi, Hajime Nawata

    Research output: Contribution to journalArticle

    36 Citations (Scopus)

    Abstract

    Reduced prostacyclin (PGI2) production by the vascular wall may play an important role in the pathogenesis of vascular lesions such as atherosclerosis. The present study was undertaken to evaluate the effect of vitamin E on the production of PGI2 and other prostaglandins (prostaglandin E2 [PGE2], thromboxane A2 [TXA2], and 15-hydroxyeicosatetraenoic acid [15-HETE]) by bovine aortic endothelial cells cultured in a high concentration of glucose (300 mg/dL). Compared with endothelial cells cultured in 100 mg/dL glucose, the production of PGI2 and other prostaglandins, except 15-HETE, was significantly reduced in cultures containing 300 mg/dL glucose when stimulated by histamine, the Ca2+ ionophore, A23187, or human plasma-derived serum (PDS). The addition of vitamin E to each stimulant significantly restored the production of PGI2, PGE2, and TXA2, products of the cyclo-oxygenase pathway, in aortic endothelial cells cultured in 300 mg/dL glucose. This effect of vitamin E on the stimulation of prostaglandin production was generally specific for d-α-tocopherol, but not for the other vitamin E analogs tested. However, vitamin E and the stimulants had no effect on the production of 15-HETE, a product of the lipoxygenase pathway. Moreover, vitamin E alone, without stimulants, did not affect prostaglandin production in cultured bovine aortic endothelial cells. These results suggest that vitamin E may restore reduced PGI2, PGE2, or TXA2 production by bovine aortic endothelial cells cultured in a high concentration of glucose. It seems likely that vitamin E may restore depressed PGI2 production by the vascular wall in hyperglycemic conditions such as those seen in patients with diabetes mellitus.

    Original languageEnglish
    Pages (from-to)613-621
    Number of pages9
    JournalMetabolism
    Volume41
    Issue number6
    DOIs
    Publication statusPublished - Jun 1992

    Fingerprint

    Epoprostenol
    Vitamin E
    Endothelial Cells
    Glucose
    Hydroxyeicosatetraenoic Acids
    Thromboxane A2
    Prostaglandins
    Dinoprostone
    Blood Vessels
    Tocopherols
    Lipoxygenase
    Ionophores
    Calcimycin
    Prostaglandin-Endoperoxide Synthases
    Histamine
    Atherosclerosis
    Diabetes Mellitus
    Serum

    All Science Journal Classification (ASJC) codes

    • Endocrinology, Diabetes and Metabolism
    • Endocrinology

    Cite this

    Vitamin E restores reduced prostacyclin synthesis in aortic endothelial cells cultured with a high concentration of glucose. / Kunisaki, Makoto; Umeda, Fumio; Inoguchi, Toyoshi; Nawata, Hajime.

    In: Metabolism, Vol. 41, No. 6, 06.1992, p. 613-621.

    Research output: Contribution to journalArticle

    Kunisaki, Makoto ; Umeda, Fumio ; Inoguchi, Toyoshi ; Nawata, Hajime. / Vitamin E restores reduced prostacyclin synthesis in aortic endothelial cells cultured with a high concentration of glucose. In: Metabolism. 1992 ; Vol. 41, No. 6. pp. 613-621.
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