Vitellogenin Incorporation into Oocytes of Rainbow Trout, Oncorhynchus mykiss, in Vitro: Effect of Hormones on Denuded Oocytes: vitellogenin/oocyte growth/insulin/thyroxine/rainbow trout

Naoki Shibata, Michiyasu Yoshikuni, Yoshitaka Nagahama

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21 Citations (Scopus)

Abstract

An in vitro culture procedure to measure vitellogenin (VTG) incorporation into oocytes without follicle cell layers was developed using oocytes of the rainbow trout, Oncorhynchus mykiss. Oocytes incorporated VTG specifically and linearly for up to 24 hr. The maximum incorporation observed was 314 μg/24 hr/oocyte, using vitellogenic (3.6 mm diameter) oocytes. The effect of hormones was examined by adding insulin, growth hormone, prolactin, gonadotropins (GTH‐I, GTH‐II), thyroid hormones, testosterone, estradiol‐17β, or 17α, 20β‐dihydroxy‐4‐pregnen‐3‐one to the medium. The results indicated that insulin and thyroxine stimulated uptake of VTG by 13% and 12%, respectively. Insulin specifically stimulated VTG incorporation and did not cause any change in background uptake of insulin. The lack of an effect of gonadotropins hormones on denuded oocytes suggests that the previously observed stimulation of VTG incorporation into follicle cell‐enclosed oocytes in vivo and in vitro by GTH‐I is most likely mediated by the somatic cells of the ovarian follicle.

Original languageEnglish
Pages (from-to)115-121
Number of pages7
JournalDevelopment, Growth & Differentiation
Volume35
Issue number1
DOIs
Publication statusPublished - Jan 1 1993

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Vitellogenins
Oncorhynchus mykiss
Thyroxine
Oocytes
Hormones
Insulin
Growth
Gonadotropins
Ovarian Follicle
In Vitro Techniques
Thyroid Hormones
Prolactin
Growth Hormone
Testosterone

All Science Journal Classification (ASJC) codes

  • Developmental Biology
  • Cell Biology

Cite this

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title = "Vitellogenin Incorporation into Oocytes of Rainbow Trout, Oncorhynchus mykiss, in Vitro: Effect of Hormones on Denuded Oocytes: vitellogenin/oocyte growth/insulin/thyroxine/rainbow trout",
abstract = "An in vitro culture procedure to measure vitellogenin (VTG) incorporation into oocytes without follicle cell layers was developed using oocytes of the rainbow trout, Oncorhynchus mykiss. Oocytes incorporated VTG specifically and linearly for up to 24 hr. The maximum incorporation observed was 314 μg/24 hr/oocyte, using vitellogenic (3.6 mm diameter) oocytes. The effect of hormones was examined by adding insulin, growth hormone, prolactin, gonadotropins (GTH‐I, GTH‐II), thyroid hormones, testosterone, estradiol‐17β, or 17α, 20β‐dihydroxy‐4‐pregnen‐3‐one to the medium. The results indicated that insulin and thyroxine stimulated uptake of VTG by 13{\%} and 12{\%}, respectively. Insulin specifically stimulated VTG incorporation and did not cause any change in background uptake of insulin. The lack of an effect of gonadotropins hormones on denuded oocytes suggests that the previously observed stimulation of VTG incorporation into follicle cell‐enclosed oocytes in vivo and in vitro by GTH‐I is most likely mediated by the somatic cells of the ovarian follicle.",
author = "Naoki Shibata and Michiyasu Yoshikuni and Yoshitaka Nagahama",
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AU - Yoshikuni, Michiyasu

AU - Nagahama, Yoshitaka

PY - 1993/1/1

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N2 - An in vitro culture procedure to measure vitellogenin (VTG) incorporation into oocytes without follicle cell layers was developed using oocytes of the rainbow trout, Oncorhynchus mykiss. Oocytes incorporated VTG specifically and linearly for up to 24 hr. The maximum incorporation observed was 314 μg/24 hr/oocyte, using vitellogenic (3.6 mm diameter) oocytes. The effect of hormones was examined by adding insulin, growth hormone, prolactin, gonadotropins (GTH‐I, GTH‐II), thyroid hormones, testosterone, estradiol‐17β, or 17α, 20β‐dihydroxy‐4‐pregnen‐3‐one to the medium. The results indicated that insulin and thyroxine stimulated uptake of VTG by 13% and 12%, respectively. Insulin specifically stimulated VTG incorporation and did not cause any change in background uptake of insulin. The lack of an effect of gonadotropins hormones on denuded oocytes suggests that the previously observed stimulation of VTG incorporation into follicle cell‐enclosed oocytes in vivo and in vitro by GTH‐I is most likely mediated by the somatic cells of the ovarian follicle.

AB - An in vitro culture procedure to measure vitellogenin (VTG) incorporation into oocytes without follicle cell layers was developed using oocytes of the rainbow trout, Oncorhynchus mykiss. Oocytes incorporated VTG specifically and linearly for up to 24 hr. The maximum incorporation observed was 314 μg/24 hr/oocyte, using vitellogenic (3.6 mm diameter) oocytes. The effect of hormones was examined by adding insulin, growth hormone, prolactin, gonadotropins (GTH‐I, GTH‐II), thyroid hormones, testosterone, estradiol‐17β, or 17α, 20β‐dihydroxy‐4‐pregnen‐3‐one to the medium. The results indicated that insulin and thyroxine stimulated uptake of VTG by 13% and 12%, respectively. Insulin specifically stimulated VTG incorporation and did not cause any change in background uptake of insulin. The lack of an effect of gonadotropins hormones on denuded oocytes suggests that the previously observed stimulation of VTG incorporation into follicle cell‐enclosed oocytes in vivo and in vitro by GTH‐I is most likely mediated by the somatic cells of the ovarian follicle.

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