Volatile and non-volatile monoterpenes produced by elicitor-stimulated Cupressus lusitanica cultured cells

Ransika De Alwis, Koki Fujita, Tatsuya Ashitani, Ken'ichi Kuroda

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Elicitor treatment initiates defense responses in cultured Cupressus lusitanica cells. In order to investigate the defense mechanism with a yeast extract elicitor, we carried out solid-phase microextraction coupled with gas chromatography for monoterpene analysis. Ten hydrocarbon monoterpenes, including high amounts of sabinene and limonene, were detected in the gas phase of the elicitor-treated cell cultures. Six oxidized monoterpenes including β-thujaplicin were also detected in the ether extract of the cells and the medium. Time-course profiles of volatile monoterpenes showed that one group of hydrocarbon monoterpenes was maximized on the second day after elicitation, while the other group was maximized on the third day. There were no oxidized monoterpenes that are structurally related to sabinene and limonene in the gas phase or cell extracts, suggesting that these compounds are produced exclusively for emission. Other monoterpenes, which are produced during later stages of elicitation, are metabolized into more complex compounds such as oxidized monoterpenes, including β-thujaplicin. Although terpinolene synthase was the principal monoterpene synthase in these cell cultures, terpinolene was detected only as a minor compound in the gas phase. The time course for terpinolene synthase activity coincided with β-thujaplicin biosynthesis. Thus, most of the terpinolene is metabolized rapidly to oxidized terpenes such as β-thujaplicin rather than emitted.

Original languageEnglish
Pages (from-to)720-728
Number of pages9
JournalJournal of Plant Physiology
Volume166
Issue number7
DOIs
Publication statusPublished - May 1 2009

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Cupressus lusitanica
Cupressus
Monoterpenes
monoterpenoids
cultured cells
Cultured Cells
terpinolene
Gases
sabinene
Hydrocarbons
Cell Extracts
gases
hydrocarbons
Cell Culture Techniques
cell culture
Solid Phase Microextraction
elicitors
Terpenes
extracts
Gas Chromatography

All Science Journal Classification (ASJC) codes

  • Physiology
  • Agronomy and Crop Science
  • Plant Science

Cite this

Volatile and non-volatile monoterpenes produced by elicitor-stimulated Cupressus lusitanica cultured cells. / De Alwis, Ransika; Fujita, Koki; Ashitani, Tatsuya; Kuroda, Ken'ichi.

In: Journal of Plant Physiology, Vol. 166, No. 7, 01.05.2009, p. 720-728.

Research output: Contribution to journalArticle

De Alwis, Ransika ; Fujita, Koki ; Ashitani, Tatsuya ; Kuroda, Ken'ichi. / Volatile and non-volatile monoterpenes produced by elicitor-stimulated Cupressus lusitanica cultured cells. In: Journal of Plant Physiology. 2009 ; Vol. 166, No. 7. pp. 720-728.
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