Voltage-dependent channels in dissociated outer hair cells of the guinea pig

Takashi Nakagawa, S. Kakehata, N. Akaike, S. Komune, T. Takasaka, T. Uemura

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Voltage-dependent channels in outer hair cells (OHCs) dissociated from the guinea pig cochlea were investigated by the use of a whole-cell patch-clamp technique. Two types of K+ current were recorded from OHCs. One was a slowly inactivating K+ current that was activated at a potential more positive than -30 mV. Another is a K+ current that was already activated at resting membrane potential. After suppressing both K+ currents, depolarizing voltage steps elicited a slowly inactivating inward current that was dependent on external Ca2+ and was indicative of an L-type Ca2+ channel in OHCs. Aminoglycoside antibiotics known to be ototoxic selectively inhibited the Ca2+ current.

Original languageEnglish
JournalEuropean Archives of Oto-Rhino-Laryngology
Volume251
Issue number1 Supplement
DOIs
Publication statusPublished - Aug 1 1994

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Outer Auditory Hair Cells
Guinea Pigs
Cochlea
Aminoglycosides
Patch-Clamp Techniques
Membrane Potentials
Anti-Bacterial Agents

All Science Journal Classification (ASJC) codes

  • Otorhinolaryngology

Cite this

Voltage-dependent channels in dissociated outer hair cells of the guinea pig. / Nakagawa, Takashi; Kakehata, S.; Akaike, N.; Komune, S.; Takasaka, T.; Uemura, T.

In: European Archives of Oto-Rhino-Laryngology, Vol. 251, No. 1 Supplement, 01.08.1994.

Research output: Contribution to journalArticle

Nakagawa, Takashi ; Kakehata, S. ; Akaike, N. ; Komune, S. ; Takasaka, T. ; Uemura, T. / Voltage-dependent channels in dissociated outer hair cells of the guinea pig. In: European Archives of Oto-Rhino-Laryngology. 1994 ; Vol. 251, No. 1 Supplement.
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