Volume-regulated anion channels serve as an auto/paracrine nucleotide release pathway in aortic endothelial cells

Kazunari Hisadome, Tetsuya Koyama, Chiwaka Kimura, Guy Droogmans, Yushi Ito, Masahiro Oike

Research output: Contribution to journalArticle

111 Citations (Scopus)

Abstract

Mechanical stress induces auto/paracrine ATP release from various cell types, but the mechanisms underlying this release are not well understood. Here we show that the release of ATP induced by hypotonic stress (HTS) in bovine aortic endothelial cells (BAECs) occurs through volume-regulated anion channels (VRAC). Various VRAC inhibitors, such as glibenclamide, verapamil, tamoxifen, and fluoxetine, suppressed the HTS-induced release of ATP, as well as the concomitant Ca2+ oscillations and NO production. They did not, however, affect Ca2+ oscillations and NO production induced by exogenously applied ATP. Extracellular ATP inhibited VRAC currents in a voltage-dependent manner: block was absent at negative potentials and was manifest at positive potentials, but decreased at highly depolarized potentials. This phenomenon could be described with a "permeating blocker model," in which ATP binds with an affinity of 1.0 ± 0.5 mM at 0 mV to a site at an electrical distance of 0.41 inside the channel. Bound ATP occludes the channel at moderate positive potentials, but permeates into the cytosol at more depolarized potentials. The triphosphate nucleotides UTP, GTP, and CTP, and the adenine nucleotide ADP, exerted a similar voltage-dependent inhibition of VRAC currents at submillimolar concentrations, which could also be described with this model. However, inhibition by ADP was less voltage sensitive, whereas adenosine did not affect VRAC currents, suggesting that the negative charges of the nucleotides are essential for their inhibitory action. The observation that high concentrations of extracellular ADP enhanced the outward component of the VRAC current in low Cl- hypotonic solution and shifted its reversal potential to negative potentials provides more direct evidence for the nucleotide permeability of VRAC. We conclude from these observations that VRAC is a nucleotide-permeable channel, which may serve as a pathway for HTS-induced ATP release in BAEC.

Original languageEnglish
Pages (from-to)511-520
Number of pages10
JournalJournal of General Physiology
Volume119
Issue number6
DOIs
Publication statusPublished - Jun 20 2002

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Anions
Nucleotides
Endothelial Cells
Adenosine Triphosphate
Osmotic Pressure
Adenosine Diphosphate
Hypotonic Solutions
Cytidine Triphosphate
Uridine Triphosphate
Mechanical Stress
Adenine Nucleotides
Glyburide
Fluoxetine
Tamoxifen
Verapamil
Guanosine Triphosphate
Cytosol
Adenosine
Permeability

All Science Journal Classification (ASJC) codes

  • Physiology

Cite this

Volume-regulated anion channels serve as an auto/paracrine nucleotide release pathway in aortic endothelial cells. / Hisadome, Kazunari; Koyama, Tetsuya; Kimura, Chiwaka; Droogmans, Guy; Ito, Yushi; Oike, Masahiro.

In: Journal of General Physiology, Vol. 119, No. 6, 20.06.2002, p. 511-520.

Research output: Contribution to journalArticle

Hisadome, Kazunari ; Koyama, Tetsuya ; Kimura, Chiwaka ; Droogmans, Guy ; Ito, Yushi ; Oike, Masahiro. / Volume-regulated anion channels serve as an auto/paracrine nucleotide release pathway in aortic endothelial cells. In: Journal of General Physiology. 2002 ; Vol. 119, No. 6. pp. 511-520.
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