Volumetric study on the protein-anesthetic binding

Michio Yamanaka, Shoji Kaneshina, Hiroshi Kamaya, Issaku Ueda

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Thermodynamic equations describing the volume behavior of protein-ligand mixtures in water were derived. In order to estimate the volume and binding parameters, the equations were combined with a Langmuir-type binding isotherm. Densities of aqueous solutions of mixtures of bovine serum albumin (BSA) and octanol (C8OH) were measured as a function of total BSA molality, mM T, at constant total C8OH molalities, mX T. The data were analyzed by the equations. The partial molar volumes at infinite dilution of BSA and C8OH, VM T,0 and VX T,0, respectively, were estimated. It was seen that VM T,0 decreases by the addition of C8OH to the solution and that VX T,0 decreases gradually with increasing mM T and approaches asymptotically to a certain value at high mM T. From the concentration dependence of VM T,0 and VX T,0, the values of the association constant K=392 kg mol-1, the maximum binding number bmax=1.9, and the volume change ΔV=-109 cm3 mol-1 were obtained for BSA-C8OH interaction in water. The negative value of ΔV indicates that the hydrophobic interaction reduces the protein volume and elevation of pressure promotes BSA-C8OH binding. These results is inconsistent with the pressure reversal of anesthesia.

Original languageEnglish
Pages (from-to)23-29
Number of pages7
JournalColloids and Surfaces B: Biointerfaces
Volume22
Issue number1
DOIs
Publication statusPublished - Jul 12 2001

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anesthetics
Anesthetics
Bovine Serum Albumin
albumins
Protein Binding
serums
proteins
Proteins
Density (specific gravity)
Dilution
Isotherms
Water
Ligands
Association reactions
Thermodynamics
anesthesia
Pressure
Octanols
Hydrophobic and Hydrophilic Interactions
water

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Colloid and Surface Chemistry
  • Physical and Theoretical Chemistry
  • Surfaces and Interfaces

Cite this

Volumetric study on the protein-anesthetic binding. / Yamanaka, Michio; Kaneshina, Shoji; Kamaya, Hiroshi; Ueda, Issaku.

In: Colloids and Surfaces B: Biointerfaces, Vol. 22, No. 1, 12.07.2001, p. 23-29.

Research output: Contribution to journalArticle

Yamanaka, Michio ; Kaneshina, Shoji ; Kamaya, Hiroshi ; Ueda, Issaku. / Volumetric study on the protein-anesthetic binding. In: Colloids and Surfaces B: Biointerfaces. 2001 ; Vol. 22, No. 1. pp. 23-29.
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