Wash-free detection of biological target using cluster formation of magnetic markers

Kazuki Akiyoshi, Takashi Yoshida, Teruyoshi Sasayama, Ahmed L. Elrefai, Misato Hara, Keiji Enpuku

Research output: Contribution to journalArticle

Abstract

We demonstrate the wash-free detection of biological targets based on the Brownian relaxation of magnetic markers. The Brownian relaxation time of bound markers was prolonged by using a cluster (or agglomerate) of bound markers that was formed via the binding reaction between markers and targets. Thus, bound and free markers were differentiated magnetically by using the difference in their relaxation times. Two types of magnetic properties, i.e., third-harmonic signal and magnetic relaxation, were used for measurement. The concentration of target was detected from the decrease in the third-harmonic signal and the increase in the magnetic-relaxation signal. We demonstrated the detection of C-reactive proteins using these two properties. In both cases, a good correlation was obtained between the measured signal and the concentration of target. It was also shown that the two methods had different concentration dependences, which will be caused mainly by the distribution of cluster size of the bound markers.

Original languageEnglish
Article number166356
JournalJournal of Magnetism and Magnetic Materials
Volume500
DOIs
Publication statusPublished - Apr 15 2020

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Magnetic relaxation
markers
Relaxation time
C-Reactive Protein
Magnetic properties
magnetic relaxation
Proteins
relaxation time
harmonics
magnetic properties
proteins

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics

Cite this

Wash-free detection of biological target using cluster formation of magnetic markers. / Akiyoshi, Kazuki; Yoshida, Takashi; Sasayama, Teruyoshi; Elrefai, Ahmed L.; Hara, Misato; Enpuku, Keiji.

In: Journal of Magnetism and Magnetic Materials, Vol. 500, 166356, 15.04.2020.

Research output: Contribution to journalArticle

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AU - Yoshida, Takashi

AU - Sasayama, Teruyoshi

AU - Elrefai, Ahmed L.

AU - Hara, Misato

AU - Enpuku, Keiji

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