WASP is involved in proliferation and differentiation of human haemopoietic progenitors in vitro

Mlchiko Kajiwara, Sliigeaki Nonoyama, Mltsuoki Ecuchi, Tomohiro Morio, Kohsuke Imai, Hiroji Okawa, Masafumi Kaneko, L. Masahiro Sako, Shouichi Oiiga, Miho Maeda, Siiigeyoshi Hmi, Hlsako Hashimito, Alsusiji Slhbuya, Hans D. Ochs, Tatsutoshi Nakahata, Jun Ichi Yata

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

The Wiskott-Aldrich syndrome (WAS) is an X-linked recessive disorder characterized by thrombocytopenia, immunodeficiency and eczema. X-linked thrombocytopenia (XLT) is a mild form of WAS with isolated thrombocytopenia. Both phenotypes are caused by mutation of the Wiskott-Aldrich syndrome protein (WASP) gene. In this study we investigated the role of WASP in the differentiation of CD34-positive (CD34+) cells isolated from the bone marrow of patients with WAS (n= 5) or with XLT (n=4). Megakaryocyte colony formation was significantly decreased in patients with WAS when compared with normal controls. The formation of granulocyte-macrophage colonies and erythroid bursts were also decreased in WAS patients. In contrast, in XLT patients, formation of all these colonies was normal. However, in vitro proplatelet formation of megakaryocytes induced by thrombopoietin was markedly decreased in both XLT and WAS. Electron microscopic examination revealed that megakaryocytes obtained from WAS or XLT patients grown in vitro had abnormal morphologic features, which seemed to be caused by defective actin cytoskeletal organization, including labyrinth-like structures of the demarcation membrane system and deviated distribution of the α-granules and demarcation membrane system. These observations indicate that WASP is involved in the proliferation and differentiation of CD34+ haemopoietic progenitor cells probably by its participation in signal transduction and in the regulation of the cytoskeleton.

Original languageEnglish
Pages (from-to)254-262
Number of pages9
JournalBritish Journal of Haematology
Volume107
Issue number2
DOIs
Publication statusPublished - Nov 22 1999
Externally publishedYes

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Hematology

Cite this

Kajiwara, M., Nonoyama, S., Ecuchi, M., Morio, T., Imai, K., Okawa, H., ... Yata, J. I. (1999). WASP is involved in proliferation and differentiation of human haemopoietic progenitors in vitro. British Journal of Haematology, 107(2), 254-262. https://doi.org/10.1046/j.1365-2141.1999.01694.x