Water and a protic ionic liquid acted as refolding additives for chemically denatured enzymes

Pankaj Attri, P. Venkatesu, Anil Kumar

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

In this communication, we present the ability of water and a protic ionic liquid, triethyl ammonium phosphate (TEAP) to act as refolding additives for the urea-induced chemical denaturated state of the two enzymes, α-chymotrypsin and succinylated Con A. We show that the enzymatic activity is regained and in certain circumstances enhanced.

Original languageEnglish
Pages (from-to)7475-7478
Number of pages4
JournalOrganic and Biomolecular Chemistry
Volume10
Issue number37
DOIs
Publication statusPublished - Oct 7 2012

Fingerprint

ammonium phosphates
Ionic Liquids
Chymotrypsin
ureas
Urea
enzymes
communication
Water
Communication
Enzymes
liquids
water
ammonium phosphate
triethyl phosphate

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physical and Theoretical Chemistry
  • Organic Chemistry

Cite this

Water and a protic ionic liquid acted as refolding additives for chemically denatured enzymes. / Attri, Pankaj; Venkatesu, P.; Kumar, Anil.

In: Organic and Biomolecular Chemistry, Vol. 10, No. 37, 07.10.2012, p. 7475-7478.

Research output: Contribution to journalArticle

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