Water-induced surface reorganization of bioscaffolds composed of an amphiphilic hyperbranched polymer

Hisao Matsuno, Masayasu Totani, Arisa Yamamoto, Masayuki Haraguchi, Masaaki Ozawa, Keiji Tanaka

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

The ability to freely control the surface of bioscaffolds in a water environment is desirable to regulate cellular behaviors in vitro. Herein, we study the surface aggregation states of scaffold films composed of a multifunctional hyperbranched polymer (HBP) with perfluorohexylethyl, carboxy, and cyano groups that was prepared using a spin-coating method. Static contact angle measurements in conjunction with X-ray photoelectron spectroscopy revealed that perfluorohexylethyl groups were segregated at the surface of the HBP film in air, and these findings were more remarkable for the film treated with thermal annealing. Once the HBP film contacted water, HBP chains reorganized at the surface to minimize the free energy, resulting in the formation of a relatively hydrophilic surface. This surface reorganization was discernably faster and more remarkable for the non-annealed HBP film than for the annealed film. As fundamental characteristics of a cellular scaffold, protein adsorption, in addition to the initial adhesion and proliferation of fibroblasts, was examined using microscopy. The amount of fibronectin adsorbed depended on the presence of thermal annealing during the scaffold preparation process. A relatively larger amount of fibronectin adsorbed to the non-annealed HBP film promoted the initial adhesion and subsequent proliferation of fibroblasts.

Original languageEnglish
Pages (from-to)1045-1053
Number of pages9
JournalPolymer Journal
Volume51
Issue number10
DOIs
Publication statusPublished - Oct 1 2019

All Science Journal Classification (ASJC) codes

  • Polymers and Plastics
  • Materials Chemistry

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