Water-soluble component in dried chrysanthemum flower stimulates tumor necrosis factor-α production by mouse macrophage-like cell line RAW264.7

Satomi Kobayashi, Naoto Ogawa, Yoshinori Fujimura, Hirofumi Tachibana, Koji Yamada

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

To clarify immunoregulatory activity of "Shiranui Himekiku" (Chrysanthemum indicam × Erigeron annus), we examined the effect on tumor necrosis factor α (TNF-α) production by mouse macrophage-like cell line RAW264.7. The dried chrysanthemum flower (CF) petals were extracted with water, and the cells were cultured in the presence of the extract. CF extract significantly enhanced TNF-α production of the cells by the dose-dependent manner. Diluted CF solution did not significantly affect the cell number and viability; however, non-diluted solution suppressed the cell proliferation and decreased the cell viability. Heating CF extract at 100°C for 30 min enhanced the activity of water extract markedly, and freeze-thawing only moderately. The TNF-α production enhancing activity of the extract was observed within 3h. These results suggest that the TNF-α production enhancing activity of CF extract can be recovered efficiently by hot water extraction and preserved stably by freezing.

Original languageEnglish
Pages (from-to)144-147
Number of pages4
JournalFood Science and Technology Research
Volume12
Issue number2
DOIs
Publication statusPublished - May 1 2006

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Chemical Engineering(all)
  • Industrial and Manufacturing Engineering
  • Marketing

Fingerprint Dive into the research topics of 'Water-soluble component in dried chrysanthemum flower stimulates tumor necrosis factor-α production by mouse macrophage-like cell line RAW264.7'. Together they form a unique fingerprint.

Cite this