We have implemented an efficient, user-friendly, and web-based "biosimulator" called BEST-KIT (Biochemical Engineering System analyzing Tool-KIT: http://helios.brs.Kyushu-u.ac.jp/~bestkit/) for analyzing a large scale nonlinear reaction networks such as metabolic pathways. BEST-KIT consists mainly of two kinds of modules, "MassAction" and "EnzymeKinetics." In this study, we have developed a new module, "MassAction++," which can construct and analyze reaction schemes represented by both mass action law (mass balance) and approximated velocity functions of enzyme kinetics at steady state. All modules in BEST-KIT are developed in Java applet style, and can be run on "any" platform machine through the web browser. The "MassAction++" module was developed in Java applet style. It can save data related to the constructed reaction scheme and time course data onto the client disk space and can load it onto the web browser.
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