Werner interacting protein 1 promotes binding of werner protein to template-primer DNA

Makoto Kanamori, Masayuki Seki, Akari Yoshimura, Toshiki Tsurimoto, Shusuke Tada, Takemi Enomoto

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Werner interacting protein 1 (WRNIP1) that is highly conserved from Escherichia coli to human was originally identified as a protein that interacts with the Werner syndrome responsible gene product (WRN). Here, human WRNIP1 and WRN are shown to bind to template-primer DNA, and WRNIP1, but not WRN, requires ATP for DNA binding. Under conditions of a limiting amount of WRN, WRNIP1 facilitated binding of WRN to DNA in a dose-dependent manner. However, WRNIP1 did not stimulate the DNA helicase activity of WRN, and WRN displaced pre-bound WRNIP1 from DNA. Functional relationships between WRNIP1 and WRN will be discussed.

Original languageEnglish
Pages (from-to)1314-1318
Number of pages5
JournalBiological and Pharmaceutical Bulletin
Volume34
Issue number8
DOIs
Publication statusPublished - Aug 2011

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science

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