Western blotting for ginseng saponins, ginsenosides using anti- ginsenoside Rb1 monoclonal antibody

Noriko Fukuda, Hiroyuki Tanaka, Yukihiro Shoyama

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

Ginsenosides separated by silica gel TLC were blotted to a polyvinylidene difluoride (PVDF) membrane which was treated with a NaIO4 solution followed by bovine serum albumine (BSA), resulting in a ginsenoside- BSA conjugate on a PVDF membrane. The blotted bands were stained with monoclonal antibody (MAb). The newly established Western blotting method was used for the determination of ginsenosides and their distribution in various Panax species.

Original languageEnglish
Pages (from-to)219-220
Number of pages2
JournalBiological and Pharmaceutical Bulletin
Volume22
Issue number2
DOIs
Publication statusPublished - Feb 1999

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science

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