Western blotting of steroidal alkaloid glycosides using monoclonal antibody against solamargine

H. Tanaka, W. Putalun, Yukihiro Shoyama

Research output: Contribution to journalArticle

Abstract

A method for the determination of solasodine glycosides using western blotting was investigated. Solasodine glycosides, separated by silica gel thin-layer chromatography, were transferred to a polyvinylidene difluoride membrane. The membrane was treated with sodium periodate solution followed by bovine serum albumin, resulting in a solasodine glycoside-bovine serum albumin conjugate. Individual spots were stained with monoclonal antibody against solamargine. Immunostaining of solasodine glycosides was more sensitive compared with other staining methods. The newly established western blotting method is extended to the distribution of solasodine glycosides in plants.

Original languageEnglish
Pages (from-to)1503-1512
Number of pages10
JournalJournal of Liquid Chromatography and Related Technologies
Volume22
Issue number10
DOIs
Publication statusPublished - Jan 1 1999

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Glycosides
Alkaloids
Western Blotting
Monoclonal Antibodies
Bovine Serum Albumin
Membranes
Thin layer chromatography
Silica Gel
Thin Layer Chromatography
Gel Chromatography
solasodine
beta-solamarine
Staining and Labeling

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Pharmaceutical Science
  • Clinical Biochemistry

Cite this

Western blotting of steroidal alkaloid glycosides using monoclonal antibody against solamargine. / Tanaka, H.; Putalun, W.; Shoyama, Yukihiro.

In: Journal of Liquid Chromatography and Related Technologies, Vol. 22, No. 10, 01.01.1999, p. 1503-1512.

Research output: Contribution to journalArticle

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