Whole-body kinetic image of a redox probe in mice using Overhauser-enhanced MRI

Nuttavut Kosem, Tatsuya Naganuma, Kazuhiro Ichikawa, Noppawan Phumala Morales, Keiji Yasukawa, Fuminori Hyodo, Ken Ichi Yamada, Hideo Utsumi

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Overhauser-enhanced MRI (OMRI) enables visualization of free radicals in animals based on dynamic nuclear polarization. Real-time data of tissue redox status gathered from kinetic images of redox-sensitive nitroxyl radical probes using OMRI provided both anatomic and physiological information. Phantom experiments demonstrated the linear correlation between the enhancement factor and the concentration of a membrane-impermeable probe, carboxy-PROXYL (3-carboxy-2,2,5,5-tetramethyl- pyrrolidine-1-oxyl). Whole-body OMRI images illustrated the in vivo kinetics of carboxy-PROXYL for 25 min. Initial distribution was observed in lung, heart, liver, and kidney, but not brain, corresponding to its minimal lipophilicity. Based on these images (pixel size, 1.33×1.33 mm; slice thickness, 50 mm), a time-concentration curve with low coefficient of variance (<0.21) was created to assess pharmacokinetic behaviors. A biexponential curve showed a distribution phase from 1 to 10 min and an elimination phase from 15 to 25 min. The α rate constant was greater than the β rate constant in ROIs, confirming that its pharmacokinetics obeyed a two-compartment model. As a noninvasive technique, combining OMRI imaging with redox probes to monitor tissue redox status may be useful in acquiring valuable information regarding organ function for preclinical and clinical studies of oxidative diseases.

Original languageEnglish
Pages (from-to)328-336
Number of pages9
JournalFree Radical Biology and Medicine
Volume53
Issue number2
DOIs
Publication statusPublished - Jul 15 2012

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Body Image
Magnetic resonance imaging
Oxidation-Reduction
Kinetics
Pharmacokinetics
Rate constants
Tissue
Liver
Free Radicals
Brain
Animals
Visualization
Pixels
Polarization
Membranes
Kidney
Imaging techniques
Lung
Experiments
3-carboxy-2,2,5,5-tetramethylpyrrolidin-1-yloxyl

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physiology (medical)

Cite this

Kosem, N., Naganuma, T., Ichikawa, K., Phumala Morales, N., Yasukawa, K., Hyodo, F., ... Utsumi, H. (2012). Whole-body kinetic image of a redox probe in mice using Overhauser-enhanced MRI. Free Radical Biology and Medicine, 53(2), 328-336. https://doi.org/10.1016/j.freeradbiomed.2012.04.026

Whole-body kinetic image of a redox probe in mice using Overhauser-enhanced MRI. / Kosem, Nuttavut; Naganuma, Tatsuya; Ichikawa, Kazuhiro; Phumala Morales, Noppawan; Yasukawa, Keiji; Hyodo, Fuminori; Yamada, Ken Ichi; Utsumi, Hideo.

In: Free Radical Biology and Medicine, Vol. 53, No. 2, 15.07.2012, p. 328-336.

Research output: Contribution to journalArticle

Kosem, N, Naganuma, T, Ichikawa, K, Phumala Morales, N, Yasukawa, K, Hyodo, F, Yamada, KI & Utsumi, H 2012, 'Whole-body kinetic image of a redox probe in mice using Overhauser-enhanced MRI', Free Radical Biology and Medicine, vol. 53, no. 2, pp. 328-336. https://doi.org/10.1016/j.freeradbiomed.2012.04.026
Kosem N, Naganuma T, Ichikawa K, Phumala Morales N, Yasukawa K, Hyodo F et al. Whole-body kinetic image of a redox probe in mice using Overhauser-enhanced MRI. Free Radical Biology and Medicine. 2012 Jul 15;53(2):328-336. https://doi.org/10.1016/j.freeradbiomed.2012.04.026
Kosem, Nuttavut ; Naganuma, Tatsuya ; Ichikawa, Kazuhiro ; Phumala Morales, Noppawan ; Yasukawa, Keiji ; Hyodo, Fuminori ; Yamada, Ken Ichi ; Utsumi, Hideo. / Whole-body kinetic image of a redox probe in mice using Overhauser-enhanced MRI. In: Free Radical Biology and Medicine. 2012 ; Vol. 53, No. 2. pp. 328-336.
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