Whole Sequence of spoIIIE-Like, Sporulation-inhibitory, and Transfer Gene (spi) in a Conjugative Plasmid, pSA1.1, of Streptomyces azureus and Detection of spi-Like Gene in the Actinomycete Chromosome

Katsumi Doi, Yasunori Ono, Eiji Yokoyama, Yuki Tsukagoe, Seiya Ogata

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The nucleotide sequence of spoIIIE-like and the sporulation-inhibitory and transfer gene (spi) in a conjugative plasmid, pSA1.1, of Streptomyces azureus were examined to detect the promoter region. Using Southern blotting and a spi fragment as probe, spi-like genes were detected in chromosomes of the host and other actinomycetes. These results suggest that there is a spi- and spoIIIE-like gene in chromosomes of some ac.

Original languageEnglish
Pages (from-to)1597-1600
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume62
Issue number8
DOIs
Publication statusPublished - Jan 1 1998

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Gene transfer
Actinobacteria
Streptomyces
Chromosomes
Plasmids
Genes
Genetic Promoter Regions
Nucleotides
Southern Blotting

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

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title = "Whole Sequence of spoIIIE-Like, Sporulation-inhibitory, and Transfer Gene (spi) in a Conjugative Plasmid, pSA1.1, of Streptomyces azureus and Detection of spi-Like Gene in the Actinomycete Chromosome",
abstract = "The nucleotide sequence of spoIIIE-like and the sporulation-inhibitory and transfer gene (spi) in a conjugative plasmid, pSA1.1, of Streptomyces azureus were examined to detect the promoter region. Using Southern blotting and a spi fragment as probe, spi-like genes were detected in chromosomes of the host and other actinomycetes. These results suggest that there is a spi- and spoIIIE-like gene in chromosomes of some ac.",
author = "Katsumi Doi and Yasunori Ono and Eiji Yokoyama and Yuki Tsukagoe and Seiya Ogata",
year = "1998",
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T1 - Whole Sequence of spoIIIE-Like, Sporulation-inhibitory, and Transfer Gene (spi) in a Conjugative Plasmid, pSA1.1, of Streptomyces azureus and Detection of spi-Like Gene in the Actinomycete Chromosome

AU - Doi, Katsumi

AU - Ono, Yasunori

AU - Yokoyama, Eiji

AU - Tsukagoe, Yuki

AU - Ogata, Seiya

PY - 1998/1/1

Y1 - 1998/1/1

N2 - The nucleotide sequence of spoIIIE-like and the sporulation-inhibitory and transfer gene (spi) in a conjugative plasmid, pSA1.1, of Streptomyces azureus were examined to detect the promoter region. Using Southern blotting and a spi fragment as probe, spi-like genes were detected in chromosomes of the host and other actinomycetes. These results suggest that there is a spi- and spoIIIE-like gene in chromosomes of some ac.

AB - The nucleotide sequence of spoIIIE-like and the sporulation-inhibitory and transfer gene (spi) in a conjugative plasmid, pSA1.1, of Streptomyces azureus were examined to detect the promoter region. Using Southern blotting and a spi fragment as probe, spi-like genes were detected in chromosomes of the host and other actinomycetes. These results suggest that there is a spi- and spoIIIE-like gene in chromosomes of some ac.

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JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

SN - 0916-8451

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