Whole Sequence of spoIIIE-Like, Sporulation-inhibitory, and Transfer Gene (spi) in a Conjugative Plasmid, pSA1.1, of Streptomyces azureus and Detection of spi-Like Gene in the Actinomycete Chromosome

Katsumi Doi, Yasunori Ono, Eiji Yokoyama, Yuki Tsukagoe, Seiya Ogata

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Abstract

The nucleotide sequence of spoIIIE-like and the sporulation-inhibitory and transfer gene (spi) in a conjugative plasmid, pSA1.1, of Streptomyces azureus were examined to detect the promoter region. Using Southern blotting and a spi fragment as probe, spi-like genes were detected in chromosomes of the host and other actinomycetes. These results suggest that there is a spi- and spoIIIE-like gene in chromosomes of some ac.

Original languageEnglish
Pages (from-to)1597-1600
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume62
Issue number8
DOIs
Publication statusPublished - Jan 1 1998

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All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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