Widely targeted metabolic profiling analysis of yeast central metabolites

Hiroko Kato, Yoshihiro Izumi, Tomohisa Hasunuma, Fumio Matsuda, Akihiko Kondo

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

A method for a widely targeted analysis was developed for the metabolic profiling of yeast central metabolism. The widely targeted method consists of 2 analyses, namely, gas chromatography-quadrupole-mass spectrometry (GC-Q-MS) operated in selected ion monitoring mode with 25m/z channels, and liquid chromatography triple-stage quadrupole (LC-QqQ)-MS operated in multiple reaction monitoring mode. This platform was set up to identify and quantify preselected 99 compounds, including sugars, sugar phosphates, organic acids, amino acids, and cofactors. The method showed good sensitivity and a wide dynamic range. For example, limits of detection for lactate and l-phenylalanine were 1.4fmol and 2.0fmol, respectively. The dynamic ranges for GC-Q-MS analysis and LC-QqQ-MS analysis were approximately 102-105 and 103-104, respectively. The metabolite profiles of 2 yeast strains, YPH499 and BY4741, under glucose-fermenting conditions were compared using the developed method. Although YPH499 and BY4741 were derived from an identical experimental strain, the profiling analysis successfully revealed a variation in metabolic phenotypes among experimental yeast strains demonstrating that the widely targeted method could be a robust and useful method for the investigation of metabolic phenotypes of Saccharomyces cerevisiae.

Original languageEnglish
Pages (from-to)665-673
Number of pages9
JournalJournal of Bioscience and Bioengineering
Volume113
Issue number5
DOIs
Publication statusPublished - May 1 2012
Externally publishedYes

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Metabolites
Yeast
Yeasts
Gas chromatography
Mass spectrometry
Sugar Phosphates
Gas Chromatography-Mass Spectrometry
Monitoring
Organic acids
Liquid chromatography
Phenylalanine
Metabolism
Sugars
Phenotype
Glucose
Amino acids
Lactic Acid
Ions
Amino Acids
Liquid Chromatography

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Widely targeted metabolic profiling analysis of yeast central metabolites. / Kato, Hiroko; Izumi, Yoshihiro; Hasunuma, Tomohisa; Matsuda, Fumio; Kondo, Akihiko.

In: Journal of Bioscience and Bioengineering, Vol. 113, No. 5, 01.05.2012, p. 665-673.

Research output: Contribution to journalArticle

Kato, Hiroko ; Izumi, Yoshihiro ; Hasunuma, Tomohisa ; Matsuda, Fumio ; Kondo, Akihiko. / Widely targeted metabolic profiling analysis of yeast central metabolites. In: Journal of Bioscience and Bioengineering. 2012 ; Vol. 113, No. 5. pp. 665-673.
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