TY - JOUR
T1 - Widely-targeted quantitative lipidomics method by supercritical fluid chromatography triple quadrupole mass spectrometry
AU - Takeda, Hiroaki
AU - Izumi, Yoshihiro
AU - Takahashi, Masatomo
AU - Paxton, Thanai
AU - Tamura, Shohei
AU - Koike, Tomonari
AU - Yu, Ying
AU - Kato, Noriko
AU - Nagase, Katsutoshi
AU - Shiomi, Masashi
AU - Bamba, Takeshi
N1 - Funding Information:
This study was partly supported by the Japan Agency for Medical Research and Development AMED-CREST Programs 18gm0910010h0203, JPMJCR1395, and JP18gm0910013 (Y.I. and T.B.); a grant from the Japan Science and Technology Agency ALCA Program of the (Y.I. and T.B.); Ministry of Education, Culture, Sports, Science, and Technology of Japan Grant-in-Aid for Scientific Research on Innovative Areas 17H06304 (Y.I. and T.B.); Japan Society for the Promotion of Science (JSPS) Grant-in-Aid for Scientific Research (C) 26505007 (T.B.); JSPS Grant-in-Aid for Scientific Research (C) 15K06557 (Y.I.); and grants from Takeda Science Foundation (T.B.), Yakult Bio-Science Foundation (T.B.), and Kieikai Research Foundation (T.B.). The study represents a portion of the dissertation submitted by H.T. to Kyushu University in partial fulfillment of the requirement for his PhD degree. The authors declare no competing financial interest. Manuscript received 26 December 2017 and in revised form 6 April 2018. Published, JLR Papers in Press, May 3, 2018 DOI https://doi.org/10.1194/jlr.D083014
Publisher Copyright:
© 2018 by the American Society.
PY - 2018
Y1 - 2018
N2 - Lipidomics, the mass spectrometry-based comprehensive analysis of lipids, has attracted attention as an analytical approach to provide novel insight into lipid metabolism and to search for biomarkers. However, an ideal method for both comprehensive and quantitative analysis of lipids has not been fully developed. Here, we have proposed a practical methodology for widely targeted quantitative lipidome analysis using supercritical fluid chromatography fast-scanning triple-quadrupole mass spectrometry (SFC/ QqQMS) and theoretically calculated a comprehensive lipid multiple reaction monitoring (MRM) library. Lipid classes can be separated by SFC with a normal-phase diethylaminebonded silica column with high resolution, high throughput, and good repeatability. Structural isomers of phospholipids can be monitored by mass spectrometric separation with fatty acyl-based MRM transitions. SFC/QqQMS analysis with an internal standard-dilution method offers quantitative information for both lipid class and individual lipid molecular species in the same lipid class. Additionally, data acquired using this method has advantages, including reduction of misidentification and acceleration of data analysis. Using the SFC/QqQMS system, alteration of plasma lipid levels in myocardial infarction-prone rabbits to the supplementation of EPA was first observed. Our developed SFC/QqQMS method represents a potentially useful tool for in-depth studies focused on complex lipid metabolism and biomarker discovery.
AB - Lipidomics, the mass spectrometry-based comprehensive analysis of lipids, has attracted attention as an analytical approach to provide novel insight into lipid metabolism and to search for biomarkers. However, an ideal method for both comprehensive and quantitative analysis of lipids has not been fully developed. Here, we have proposed a practical methodology for widely targeted quantitative lipidome analysis using supercritical fluid chromatography fast-scanning triple-quadrupole mass spectrometry (SFC/ QqQMS) and theoretically calculated a comprehensive lipid multiple reaction monitoring (MRM) library. Lipid classes can be separated by SFC with a normal-phase diethylaminebonded silica column with high resolution, high throughput, and good repeatability. Structural isomers of phospholipids can be monitored by mass spectrometric separation with fatty acyl-based MRM transitions. SFC/QqQMS analysis with an internal standard-dilution method offers quantitative information for both lipid class and individual lipid molecular species in the same lipid class. Additionally, data acquired using this method has advantages, including reduction of misidentification and acceleration of data analysis. Using the SFC/QqQMS system, alteration of plasma lipid levels in myocardial infarction-prone rabbits to the supplementation of EPA was first observed. Our developed SFC/QqQMS method represents a potentially useful tool for in-depth studies focused on complex lipid metabolism and biomarker discovery.
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U2 - 10.1194/jlr.D083014
DO - 10.1194/jlr.D083014
M3 - Article
C2 - 29724780
AN - SCOPUS:85049503691
VL - 59
SP - 1283
EP - 1293
JO - Journal of Lipid Research
JF - Journal of Lipid Research
SN - 0022-2275
IS - 7
ER -