Abstract
We report here a method that allows fast, efficient, and low-cost screening for gene function in the vascular system of the vertebrate embryo. Through intracardiac delivery of nucleic acids optimally compacted by a specific cationic lipid, we are able to induce in vivo endothelial cell-specific gain-of-function during development of the vascular network in the chick embryo. When the nucleic acids are delivered during the period of intraembryonic hematopoiesis, aortic hemangioblasts, the forerunners of the hematopoietic stem cells known to derive from the aortic endothelium, are also labeled. Similarly, we show that siRNA could be used to induce loss-of-function in vascular endothelial cells. This gene transfer technique was also applied to the mouse embryo with a high efficiency. The present method allows large-scale analysis and may represent a new and versatile tool for functional genomics.
| Original language | English |
|---|---|
| Pages (from-to) | 105-114 |
| Number of pages | 10 |
| Journal | Developmental Dynamics |
| Volume | 235 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 1 2006 |
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All Science Journal Classification (ASJC) codes
- Developmental Biology
Cite this
Widespread lipoplex-mediated gene transfer to vascular endothelial cells and hemangioblasts in the vertebrate embryo. / Bollérot, Karine; Sugiyama, Daisuke; Escriou, Virginie; Gautier, Rodolphe; Tozer, Samuel; Scherman, Daniel; Jaffredo, Thierry.
In: Developmental Dynamics, Vol. 235, No. 1, 01.01.2006, p. 105-114.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Widespread lipoplex-mediated gene transfer to vascular endothelial cells and hemangioblasts in the vertebrate embryo
AU - Bollérot, Karine
AU - Sugiyama, Daisuke
AU - Escriou, Virginie
AU - Gautier, Rodolphe
AU - Tozer, Samuel
AU - Scherman, Daniel
AU - Jaffredo, Thierry
PY - 2006/1/1
Y1 - 2006/1/1
N2 - We report here a method that allows fast, efficient, and low-cost screening for gene function in the vascular system of the vertebrate embryo. Through intracardiac delivery of nucleic acids optimally compacted by a specific cationic lipid, we are able to induce in vivo endothelial cell-specific gain-of-function during development of the vascular network in the chick embryo. When the nucleic acids are delivered during the period of intraembryonic hematopoiesis, aortic hemangioblasts, the forerunners of the hematopoietic stem cells known to derive from the aortic endothelium, are also labeled. Similarly, we show that siRNA could be used to induce loss-of-function in vascular endothelial cells. This gene transfer technique was also applied to the mouse embryo with a high efficiency. The present method allows large-scale analysis and may represent a new and versatile tool for functional genomics.
AB - We report here a method that allows fast, efficient, and low-cost screening for gene function in the vascular system of the vertebrate embryo. Through intracardiac delivery of nucleic acids optimally compacted by a specific cationic lipid, we are able to induce in vivo endothelial cell-specific gain-of-function during development of the vascular network in the chick embryo. When the nucleic acids are delivered during the period of intraembryonic hematopoiesis, aortic hemangioblasts, the forerunners of the hematopoietic stem cells known to derive from the aortic endothelium, are also labeled. Similarly, we show that siRNA could be used to induce loss-of-function in vascular endothelial cells. This gene transfer technique was also applied to the mouse embryo with a high efficiency. The present method allows large-scale analysis and may represent a new and versatile tool for functional genomics.
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UR - http://www.scopus.com/inward/citedby.url?scp=33644988025&partnerID=8YFLogxK
U2 - 10.1002/dvdy.20579
DO - 10.1002/dvdy.20579
M3 - Article
VL - 235
SP - 105
EP - 114
JO - Developmental Dynamics
JF - Developmental Dynamics
SN - 1058-8388
IS - 1
ER -