X-Ray crystal structure of the DNA-binding domain of response regulator WalR essential to the cell viability of staphylococcus aureus and interaction with target DNA

Akihiro Doi, Toshihide Okajima, Yasuhiro Gotoh, Katsuyuki Tanizawa, Ryutaro Utsumi

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A bacterial two-component signal transduction system, WalK/WalR, is essential to the cell viability of Gram-positive bacteria and is therefore a potential target for the development of a new class of antibiotics. We have solved the X-ray crystal structure of the DNA-binding domain of the response regulator WalR (WalRc) from a Gram-positive pathogen Staphylococcus aureus, currently causing serious problems in public health through the acquisition of multi-drug resistance. The structure contains a winged helix-turn-helix motif and closely resembles those of WalRs of Bacillus subtilis and Enterococcus faecalis, and also that of PhoB of Escherichia coli. Gel mobility shift assays with mutant WalRs revealed specific interactions of WalR with the target DNA, as elaborated by in silico modeling of the WalRc-DNA complex.

Original languageEnglish
Pages (from-to)1901-1907
Number of pages7
JournalBioscience, Biotechnology and Biochemistry
Volume74
Issue number9
DOIs
Publication statusPublished - Oct 8 2010

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Staphylococcus aureus
Cell Survival
Crystal structure
Cells
X-Rays
X rays
DNA
Helix-Turn-Helix Motifs
Signal transduction
Enterococcus faecalis
Gram-Positive Bacteria
Multiple Drug Resistance
Electrophoretic Mobility Shift Assay
Public health
Pathogens
Bacilli
Bacillus subtilis
Computer Simulation
Escherichia coli
Assays

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

X-Ray crystal structure of the DNA-binding domain of response regulator WalR essential to the cell viability of staphylococcus aureus and interaction with target DNA. / Doi, Akihiro; Okajima, Toshihide; Gotoh, Yasuhiro; Tanizawa, Katsuyuki; Utsumi, Ryutaro.

In: Bioscience, Biotechnology and Biochemistry, Vol. 74, No. 9, 08.10.2010, p. 1901-1907.

Research output: Contribution to journalArticle

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