Xylooligosaccharide fermentation with Leuconostoc lactis

Hitomi Ohara, Michiko Owaki, Kenji Sonomoto

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Strains of Leuconostoc lactis SHO-47 and Le. lactis SHO-54, producing the clinically useful enzyme NAD-specific 6-phosphoglucanate dehydrogenase, were cultivated with a hydrolyzed birch wood xylan as the unique carbon source to produce D-lactic acid for poly(D-lactic acid). In addition to the strains SHO-47 and SHO-54, Lactococcus lactis IO-1, well known as a good xylose-utilizing lactic acid bacterium, was used as a control to confirm the extent of hemicellulose hydrolysis. The fermentation time for lactic acid of strains SHO-47 and SHO-54 was 12 h, and produced respectively 2.3 and 2.2 g/l lactic acid from 8.5 g/l hydrolyzed xylan, whereas the fermentation time of strain IO-1 was 21 h, and produced 1.3 g/l lactic acid. Xylooligosaccharides from xylobiose to xylohexose were utilized more rapidly than xylose in the cultures of strains SHO-47 and SHO-54. However, xylose concentration increased temporarily and then decreased in the culture of strain IO-1. On the other hand, xylooligosaccharides larger than xyloheptaose were not utilized by these three strains. The xylosidase activities of SHO-47, SHO-54, and IO-1 were induced by xylose or a mixture of xylobiose and xylotriose. The xylosidases of these three strains were localized in their cytoplasm.

Original languageEnglish
Pages (from-to)415-420
Number of pages6
JournalJournal of Bioscience and Bioengineering
Volume101
Issue number5
DOIs
Publication statusPublished - May 1 2006

Fingerprint

Leuconostoc
Lactic acid
Fermentation
Lactic Acid
Xylose
Xylosidases
Xylans
Betula
Lactococcus lactis
NAD
xylooligosaccharide
Hydrolysis
Bacteria
Wood
Oxidoreductases
Cytoplasm
Carbon
Enzymes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Xylooligosaccharide fermentation with Leuconostoc lactis. / Ohara, Hitomi; Owaki, Michiko; Sonomoto, Kenji.

In: Journal of Bioscience and Bioengineering, Vol. 101, No. 5, 01.05.2006, p. 415-420.

Research output: Contribution to journalArticle

Ohara, Hitomi ; Owaki, Michiko ; Sonomoto, Kenji. / Xylooligosaccharide fermentation with Leuconostoc lactis. In: Journal of Bioscience and Bioengineering. 2006 ; Vol. 101, No. 5. pp. 415-420.
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