Y-box binding protein-1 regulates cell proliferation and is associated with clinical outcomes of osteosarcoma

Y. Fujiwara-Okada, Y. Matsumoto, J. Fukushi, N. Setsu, S. Matsuura, S. Kamura, T. Fujiwara, K. Iida, M. Hatano, A. Nabeshima, H. Yamada, M. Ono, Y. Oda, Y. Iwamoto

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Background:Prognosis of osteosarcoma (OS) with distant metastasis and local recurrence is still poor. Y-box binding protein-1 (YB-1) is a multifunctional protein that can act as a regulator of transcription and translation and its high expression of YB-1 protein was observed in OS, however, the role of YB-1 in OS remains unclear.Methods:Y-box binding protein-1 expression in OS cells was inhibited by specific small interfering RNAs to YB-1 (si-YB-1). The effects of si-YB-1 in cell proliferation and cell cycle transition in OS cells were analysed in vitro and in vivo. The association of nuclear expression of YB-1 and clinical prognosis was also investigated by immunohistochemistry.Results: Proliferation of OS cell was suppressed by si-YB-1 in vivo and in vitro. The expression of cyclin D1 and cyclin A were also decreased by si-YB-1. In addition, si-YB-1 induced G1/S arrest with decreased cyclin D1 and cyclin A in OS cell lines. Direct binding of YB-1 in OS cell lines was also observed. Finally, the nuclear expression of YB-1 was significantly related to the poorer overall survival in OS patients.Conclusion:Y-box binding protein-1 would regulate cell cycle progression at G1/S and tumour growth in human OS cells in vitro and in vivo. Nuclear expression of YB-1 was closely associated with the prognosis of OS, thus, YB-1 simultaneously could be a potent molecular target and prognostic biomarker for OS.

Original languageEnglish
Pages (from-to)836-847
Number of pages12
JournalBritish journal of cancer
Volume108
Issue number4
DOIs
Publication statusPublished - Mar 5 2013

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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