TY - JOUR
T1 - Y-box binding protein-1 regulates cell proliferation and is associated with clinical outcomes of osteosarcoma
AU - Fujiwara-Okada, Y.
AU - Matsumoto, Y.
AU - Fukushi, J.
AU - Setsu, N.
AU - Matsuura, S.
AU - Kamura, S.
AU - Fujiwara, T.
AU - Iida, K.
AU - Hatano, M.
AU - Nabeshima, A.
AU - Yamada, H.
AU - Ono, M.
AU - Oda, Y.
AU - Iwamoto, Y.
N1 - Funding Information:
We thank Dr Masaki Shiota for technical advice and Dr Junji Kishimoto for helpful suggestions on statistics. This work was supported by a Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science, and a Grant-in-Aid for Clinical Research Evidence-Based Medicine and Cancer Research from the Ministry of Health, Labour and Welfare of Japan (#19390397 and #23592192).
PY - 2013/3/5
Y1 - 2013/3/5
N2 - Background:Prognosis of osteosarcoma (OS) with distant metastasis and local recurrence is still poor. Y-box binding protein-1 (YB-1) is a multifunctional protein that can act as a regulator of transcription and translation and its high expression of YB-1 protein was observed in OS, however, the role of YB-1 in OS remains unclear.Methods:Y-box binding protein-1 expression in OS cells was inhibited by specific small interfering RNAs to YB-1 (si-YB-1). The effects of si-YB-1 in cell proliferation and cell cycle transition in OS cells were analysed in vitro and in vivo. The association of nuclear expression of YB-1 and clinical prognosis was also investigated by immunohistochemistry.Results: Proliferation of OS cell was suppressed by si-YB-1 in vivo and in vitro. The expression of cyclin D1 and cyclin A were also decreased by si-YB-1. In addition, si-YB-1 induced G1/S arrest with decreased cyclin D1 and cyclin A in OS cell lines. Direct binding of YB-1 in OS cell lines was also observed. Finally, the nuclear expression of YB-1 was significantly related to the poorer overall survival in OS patients.Conclusion:Y-box binding protein-1 would regulate cell cycle progression at G1/S and tumour growth in human OS cells in vitro and in vivo. Nuclear expression of YB-1 was closely associated with the prognosis of OS, thus, YB-1 simultaneously could be a potent molecular target and prognostic biomarker for OS.
AB - Background:Prognosis of osteosarcoma (OS) with distant metastasis and local recurrence is still poor. Y-box binding protein-1 (YB-1) is a multifunctional protein that can act as a regulator of transcription and translation and its high expression of YB-1 protein was observed in OS, however, the role of YB-1 in OS remains unclear.Methods:Y-box binding protein-1 expression in OS cells was inhibited by specific small interfering RNAs to YB-1 (si-YB-1). The effects of si-YB-1 in cell proliferation and cell cycle transition in OS cells were analysed in vitro and in vivo. The association of nuclear expression of YB-1 and clinical prognosis was also investigated by immunohistochemistry.Results: Proliferation of OS cell was suppressed by si-YB-1 in vivo and in vitro. The expression of cyclin D1 and cyclin A were also decreased by si-YB-1. In addition, si-YB-1 induced G1/S arrest with decreased cyclin D1 and cyclin A in OS cell lines. Direct binding of YB-1 in OS cell lines was also observed. Finally, the nuclear expression of YB-1 was significantly related to the poorer overall survival in OS patients.Conclusion:Y-box binding protein-1 would regulate cell cycle progression at G1/S and tumour growth in human OS cells in vitro and in vivo. Nuclear expression of YB-1 was closely associated with the prognosis of OS, thus, YB-1 simultaneously could be a potent molecular target and prognostic biomarker for OS.
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U2 - 10.1038/bjc.2012.579
DO - 10.1038/bjc.2012.579
M3 - Article
C2 - 23462806
AN - SCOPUS:84875200378
SN - 0007-0920
VL - 108
SP - 836
EP - 847
JO - British Journal of Cancer
JF - British Journal of Cancer
IS - 4
ER -
