Yeast-based recombineering of DNA fragments into plant transformation vectors by one-step transformation

Yukio Nagano, Syoko Takao, Takahiro Kudo, Ei'ichi Iizasa, Toyoaki Anai

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

T-DNA binary vectors are often used in plant transformation experiments. Because they are usually very large and have few restriction sites suitable for DNA ligation reactions, cloning DNA fragments into these vectors is difficult. We provide herein an alternative to cloning DNA fragments into very large vectors. Our yeast-based recombineering method enables DNA fragments to be cloned into certain types of T-DNA binary vectors by one-step transformation without the requirement of specific recombination sites or precisely positioned restriction ends, thus making the cloning process more flexible. Moreover, this method is inexpensive and is applicable to multifragment cloning.

Original languageEnglish
Pages (from-to)2111-2117
Number of pages7
JournalPlant Cell Reports
Volume26
Issue number12
DOIs
Publication statusPublished - Dec 2007
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Plant Science

Fingerprint

Dive into the research topics of 'Yeast-based recombineering of DNA fragments into plant transformation vectors by one-step transformation'. Together they form a unique fingerprint.

Cite this