Yeast flocculin: Methods for quantitative analysis of flocculation in yeast cells

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Flocculation, the clump forming property of yeast, has long been appreciated in breweries and utilized as an off-cost method to enable the reuse of yeast cells. Members of the flocculin protein family were identified as the adherent proteins on the cell surface responsible for flocculation, and their properties have been investigated. Crystal structures of the adhesion domain of flocculins revealed their unique mode of ligand binding where a calcium ion is located in the middle of the interface between flocculin and the interacting sugar. Here we describe the most commonly used flocculation assay. The method is simple and easy, yet it is the most direct and reliable assay to evaluate the flocculation cellular phenotype.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages437-444
Number of pages8
DOIs
Publication statusPublished - 2020

Publication series

NameMethods in Molecular Biology
Volume2132
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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  • Cite this

    Maekawa, H., & Takegawa, K. (2020). Yeast flocculin: Methods for quantitative analysis of flocculation in yeast cells. In Methods in Molecular Biology (pp. 437-444). (Methods in Molecular Biology; Vol. 2132). Humana Press Inc.. https://doi.org/10.1007/978-1-0716-0430-4_42