Yeast [PSI+] "prions" that are crosstransmissible and susceptible beyond a species barrier through a quasi-prion state

Toru Nakayashiki, Kanae Ebihara, Hideo Bannai, Yoshikazu Nakamura

Research output: Contribution to journalArticle

98 Citations (Scopus)

Abstract

The yeast [PSI+] element represents an aggregated form of release factor Sup35p and is inherited by a prion mechanism. A "species barrier" prevents crosstransmission of the [PSI+] state between heterotypic Sup35p "prions." Kluyveromyces lactis and Yarrowia lipolytica Sup35 proteins, however, show interspecies [PSI+] transmissibility and susceptibility and a high spontaneous propagation rate. Cross-seeding was visualized by coaggregation of differential fluorescence probes fused to heterotypic Sup35 proteins. This coaggregation state, referred to as a "quasi-prion" state, can be stably maintained as a heritable [PSI+] element composed of heterologous Sup35 proteins. K. lactis Sup35p was capable of forming [PSI+] elements not only in S. cerevisiae but in K. lactis. These two Sup35 proteins contain unique multiple imperfect oligopeptide repeats responsible for crosstransmission and high spontaneous propagation of novel [PSI+] elements.

Original languageEnglish
Pages (from-to)1121-1130
Number of pages10
JournalMolecular Cell
Volume7
Issue number6
DOIs
Publication statusPublished - Jan 1 2001
Externally publishedYes

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Prions
Yeasts
Proteins
Yarrowia
Kluyveromyces
Oligopeptides
Saccharomyces cerevisiae
Fluorescence

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Yeast [PSI+] "prions" that are crosstransmissible and susceptible beyond a species barrier through a quasi-prion state. / Nakayashiki, Toru; Ebihara, Kanae; Bannai, Hideo; Nakamura, Yoshikazu.

In: Molecular Cell, Vol. 7, No. 6, 01.01.2001, p. 1121-1130.

Research output: Contribution to journalArticle

Nakayashiki, Toru ; Ebihara, Kanae ; Bannai, Hideo ; Nakamura, Yoshikazu. / Yeast [PSI+] "prions" that are crosstransmissible and susceptible beyond a species barrier through a quasi-prion state. In: Molecular Cell. 2001 ; Vol. 7, No. 6. pp. 1121-1130.
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