yellow and ebony are the responsible genes for the larval color mutants of the silkworm Bombyx mori

Ryo Futahashi, Jotaro Sato, Yan Meng, Shun Okamoto, Takaaki Daimon, Kimiko Yamamoto, Yoshitaka Suetsugu, Junko Narukawa, Hirokazu Takahashi, Yutaka Banno, Susumu Katsuma, Toru Shimada, Kazuei Mita, Haruhiko Fujiwara

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

Many larval color mutants have been obtained in the silkworm Bombyx mori. Mapping of melanin-synthesis genes on the Bombyx linkage map revealed that yellow and ebony genes were located near the chocolate (ch) and sooty (so) loci, respectively. In the ch mutants, body color of neonate larvae and the body markings of elder instar larvae are reddish brown instead of normal black. Mutations at the so locus produce smoky larvae and black pupae. F2 linkage analyses showed that sequence polymorphisms of yellow and ebony genes perfectly cosegregated with the ch and so mutant phenotypes, respectively. Both yellow and ebony were expressed in the epidermis during the molting period when cuticular pigmentation occurred. The spatial expression pattern of yellow transcripts coincided with the larval black markings. In the ch mutants, nonsense mutations of the yellow gene were detected, whereas large deletions of the ebony ORF were detected in the so mutants. These results indicate that yellow and ebony are the responsible genes for the ch and so loci, respectively. Our findings suggest that Yellow promotes melanization, whereas Ebony inhibits melanization in Lepidoptera and that melanin-synthesis enzymes play a critical role in the lepidopteran larval color pattern.

Original languageEnglish
Pages (from-to)1995-2005
Number of pages11
JournalGenetics
Volume180
Issue number4
DOIs
Publication statusPublished - Dec 1 2008

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Bombyx
Color
Larva
Genes
Melanins
Molting
Pupa
Lepidoptera
Nonsense Codon
Pigmentation
Epidermis
Open Reading Frames
Sequence Analysis
Chocolate
Phenotype
Mutation
Enzymes

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Futahashi, R., Sato, J., Meng, Y., Okamoto, S., Daimon, T., Yamamoto, K., ... Fujiwara, H. (2008). yellow and ebony are the responsible genes for the larval color mutants of the silkworm Bombyx mori. Genetics, 180(4), 1995-2005. https://doi.org/10.1534/genetics.108.096388

yellow and ebony are the responsible genes for the larval color mutants of the silkworm Bombyx mori. / Futahashi, Ryo; Sato, Jotaro; Meng, Yan; Okamoto, Shun; Daimon, Takaaki; Yamamoto, Kimiko; Suetsugu, Yoshitaka; Narukawa, Junko; Takahashi, Hirokazu; Banno, Yutaka; Katsuma, Susumu; Shimada, Toru; Mita, Kazuei; Fujiwara, Haruhiko.

In: Genetics, Vol. 180, No. 4, 01.12.2008, p. 1995-2005.

Research output: Contribution to journalArticle

Futahashi, R, Sato, J, Meng, Y, Okamoto, S, Daimon, T, Yamamoto, K, Suetsugu, Y, Narukawa, J, Takahashi, H, Banno, Y, Katsuma, S, Shimada, T, Mita, K & Fujiwara, H 2008, 'yellow and ebony are the responsible genes for the larval color mutants of the silkworm Bombyx mori', Genetics, vol. 180, no. 4, pp. 1995-2005. https://doi.org/10.1534/genetics.108.096388
Futahashi R, Sato J, Meng Y, Okamoto S, Daimon T, Yamamoto K et al. yellow and ebony are the responsible genes for the larval color mutants of the silkworm Bombyx mori. Genetics. 2008 Dec 1;180(4):1995-2005. https://doi.org/10.1534/genetics.108.096388
Futahashi, Ryo ; Sato, Jotaro ; Meng, Yan ; Okamoto, Shun ; Daimon, Takaaki ; Yamamoto, Kimiko ; Suetsugu, Yoshitaka ; Narukawa, Junko ; Takahashi, Hirokazu ; Banno, Yutaka ; Katsuma, Susumu ; Shimada, Toru ; Mita, Kazuei ; Fujiwara, Haruhiko. / yellow and ebony are the responsible genes for the larval color mutants of the silkworm Bombyx mori. In: Genetics. 2008 ; Vol. 180, No. 4. pp. 1995-2005.
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