Yolk of a "scanty vitellin" mutant, vit, of Bombyx mori is lacking in vitellin and 30 kDa proteins

Kazuhiro Fujikawa, Yutaka Kawaguchi, Yutaka Banno, Katsumi Koga, Hiroshi Doira

Research output: Contribution to journalArticle

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Abstract

A novel mutant named "scanty vitellin" (vit) of Bombyx mori, induced by N-methyl-N-nitrosourea, was analyzed for yolk proteins by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. The ovary of vit/vit pupae was lacking in vitellin and the 30 kDa proteins that are yolk constituents synthesized outside the ovary. However, the vit/vit ovary had a normal amount of the egg-specific protein, a yolk component synthesized by the ovarian follicular epithelial cells. On the other hand, vitellogenin and the 30 kDa proteins, which are the precursors of yolk proteins, were abundant in the haemolymph of vit/vit female pupae. These results indicate that the mutant gene interferes with the uptake of the extraovarian yolk precursors into the oocyte.

Original languageEnglish
Pages (from-to)585-589
Number of pages5
JournalComparative Biochemistry and Physiology -- Part A: Physiology
Volume112
Issue number3-4
DOIs
Publication statusPublished - Jan 1 1995

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Vitellins
Bombyx
Egg Proteins
Proteins
Ovary
Pupa
Vitellogenins
Methylnitrosourea
Protein Precursors
Hemolymph
Electrophoresis
Sodium Dodecyl Sulfate
Oocytes
Polyacrylamide Gel Electrophoresis
Genes
Epithelial Cells

All Science Journal Classification (ASJC) codes

  • Physiology

Cite this

Yolk of a "scanty vitellin" mutant, vit, of Bombyx mori is lacking in vitellin and 30 kDa proteins. / Fujikawa, Kazuhiro; Kawaguchi, Yutaka; Banno, Yutaka; Koga, Katsumi; Doira, Hiroshi.

In: Comparative Biochemistry and Physiology -- Part A: Physiology, Vol. 112, No. 3-4, 01.01.1995, p. 585-589.

Research output: Contribution to journalArticle

Fujikawa, Kazuhiro ; Kawaguchi, Yutaka ; Banno, Yutaka ; Koga, Katsumi ; Doira, Hiroshi. / Yolk of a "scanty vitellin" mutant, vit, of Bombyx mori is lacking in vitellin and 30 kDa proteins. In: Comparative Biochemistry and Physiology -- Part A: Physiology. 1995 ; Vol. 112, No. 3-4. pp. 585-589.
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