Zebrafish ftz-f1 gene has two promoters, is alternatively spliced, and is expressed in digestive organs

Wen Wen Lin, Hsin Wei Wang, Cristina Sum, Dong Liu, Choy L. Hew, Bon Chu Chung

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Fushi-tarazu Factor-1 (FTZ-F1) is a family of nuclear receptors involved in various developmental processes. We have cloned a zebrafish FTZ-F1 gene, termed ff1, which belongs to the fetoprotein transcription factor/liver receptor homologue-1 (FTF/LRH-1) subgroup of the FTZ-F1 family. Four transcripts arise as a result of differential promoter usage and alternative splicing at the 3'-most exons. The longer transcript, form A, encodes a transcriptional activator. The shorter transcript, form B, lacks the activation domain, and hence could not activate transcription. The difference in promoter usage generates FF1 proteins with different N-terminal sequences. All four transcripts appear to be expressed in most of the adult tissues, whereas, during embryo development, the IIA form is the predominant transcript. Reverse transcriptase-PCR and in situ hybridization experiments showed that the ff1 transcript is expressed in the hypothalamus, spinal cord, mandibular arch and digestive organs, including pancreas. liver, and intestine. The expression of ff1 in the digestive organs implies its function in gut development.

Original languageEnglish
Pages (from-to)439-446
Number of pages8
JournalBiochemical Journal
Volume348
Issue number2
DOIs
Publication statusPublished - Jun 1 2000

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Zebrafish
Liver
Genes
Steroidogenic Factor 1
RNA-Directed DNA Polymerase
Alternative Splicing
Arches
Transcription
Cytoplasmic and Nuclear Receptors
Reverse Transcriptase Polymerase Chain Reaction
Hypothalamus
Intestines
Embryonic Development
In Situ Hybridization
Pancreas
Exons
Spinal Cord
Chemical activation
Tissue
Proteins

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Zebrafish ftz-f1 gene has two promoters, is alternatively spliced, and is expressed in digestive organs. / Lin, Wen Wen; Wang, Hsin Wei; Sum, Cristina; Liu, Dong; Hew, Choy L.; Chung, Bon Chu.

In: Biochemical Journal, Vol. 348, No. 2, 01.06.2000, p. 439-446.

Research output: Contribution to journalArticle

Lin, Wen Wen ; Wang, Hsin Wei ; Sum, Cristina ; Liu, Dong ; Hew, Choy L. ; Chung, Bon Chu. / Zebrafish ftz-f1 gene has two promoters, is alternatively spliced, and is expressed in digestive organs. In: Biochemical Journal. 2000 ; Vol. 348, No. 2. pp. 439-446.
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