Zebrafish monosex population reveals female dominance in sex determination and earliest events of gonad differentiation

Sok Keng Tong, Hwei Jan Hsu, Bon Chu Chung

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

The zebrafish is a popular model for genetic analysis and its sex differentiation has been the focus of attention for breeding purposes. Despite numerous efforts, very little is known about the mechanism of zebrafish sex determination. The lack of discernible sex chromosomes and the difficulty of distinguishing the sex of juvenile fish are two major obstacles that hamper the progress in such studies. To alleviate these problems, we have developed a scheme involving methyltestosterone treatment followed by natural mating to generate fish with predictable sex trait. Female F1 fish that gave rise to all-female offspring were generated. This predictable sex trait enables characterization of gonadal development in juvenile fish by histological examination and gene expression analysis. We found the first sign of zebrafish sex differentiation to be ovarian gonocyte proliferation and differentiation at 10 to 12 days post-fertilization (dpf). Somatic genes were expressed indifferently at 10 to 17 dpf, and then became sexually dimorphic at three weeks. This result indicates clear distinction of male and female gonads derived independently from primordial gonads. We classified the earliest stages of zebrafish sex determination into the initial preparation followed by female germ cell growth, oocyte differentiation, and somatic differentiation. Our genetic selection scheme matches the prediction that female-dominant genetic factors are required to determine zebrafish sex.

Original languageEnglish
Pages (from-to)849-856
Number of pages8
JournalDevelopmental Biology
Volume344
Issue number2
DOIs
Publication statusPublished - Jan 1 2010

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Gonads
Zebrafish
Fishes
Population
Sex Differentiation
Fertilization
Methyltestosterone
Sex Chromosomes
Genetic Selection
Genetic Models
Germ Cells
Oocytes
Breeding
Gene Expression
Growth
Genes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Cite this

Zebrafish monosex population reveals female dominance in sex determination and earliest events of gonad differentiation. / Tong, Sok Keng; Hsu, Hwei Jan; Chung, Bon Chu.

In: Developmental Biology, Vol. 344, No. 2, 01.01.2010, p. 849-856.

Research output: Contribution to journalArticle

Tong, Sok Keng ; Hsu, Hwei Jan ; Chung, Bon Chu. / Zebrafish monosex population reveals female dominance in sex determination and earliest events of gonad differentiation. In: Developmental Biology. 2010 ; Vol. 344, No. 2. pp. 849-856.
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