TY - JOUR
T1 - ZFAT expression in B and T lymphocytes and identification of ZFAT-regulated genes
AU - Koyanagi, Midori
AU - Nakabayashi, Kazuhiko
AU - Fujimoto, Takahiro
AU - Gu, Ning
AU - Baba, Iwai
AU - Takashima, Yasuo
AU - Doi, Keiko
AU - Harada, Haruhito
AU - Kato, Norihiro
AU - Sasazuki, Takehiko
AU - Shirasawa, Senji
N1 - Funding Information:
This work was supported by a grant from the Genome Network Project; a Grant-in-Aid for Scientific Research on Priority Areas “Applied Genomics” from the Ministry of Education, Culture, Sports, Science, and Technology; and a Grant-in-Aid for Scientific Research (B) from the Japan Society for the Promotion of the Science.
PY - 2008/5
Y1 - 2008/5
N2 - The human ZFAT gene encodes a 1243-amino-acid protein containing one AT hook and 18 C2H2 zinc finger domains, which are highly conserved among ZFAT orthologues from fish to mammalian species. Consistent with the presence of multiple predicted nuclear localization signals, endogenous ZFAT protein was found to be localized to the nucleus. In the mouse tissues examined by Western blotting, ZFAT was found to be expressed in thymus, spleen, and lymph nodes, but not in other tissues, including bone marrow. Furthermore, ZFAT protein was found to be up-regulated during the transition from CD4-CD8- to CD4+CD8+ thymocytes and to be expressed only in B and T lymphocytes in peripheral lymphoid tissues. Expression array analyses demonstrated that genes that are down-regulated upon ZFAT overexpression in mouse Ba/F3 cells are significantly enriched for those functionally related to immune responses. These results suggest that ZFAT functions as a critical transcriptional regulator in B and T lymphocytes.
AB - The human ZFAT gene encodes a 1243-amino-acid protein containing one AT hook and 18 C2H2 zinc finger domains, which are highly conserved among ZFAT orthologues from fish to mammalian species. Consistent with the presence of multiple predicted nuclear localization signals, endogenous ZFAT protein was found to be localized to the nucleus. In the mouse tissues examined by Western blotting, ZFAT was found to be expressed in thymus, spleen, and lymph nodes, but not in other tissues, including bone marrow. Furthermore, ZFAT protein was found to be up-regulated during the transition from CD4-CD8- to CD4+CD8+ thymocytes and to be expressed only in B and T lymphocytes in peripheral lymphoid tissues. Expression array analyses demonstrated that genes that are down-regulated upon ZFAT overexpression in mouse Ba/F3 cells are significantly enriched for those functionally related to immune responses. These results suggest that ZFAT functions as a critical transcriptional regulator in B and T lymphocytes.
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U2 - 10.1016/j.ygeno.2008.01.009
DO - 10.1016/j.ygeno.2008.01.009
M3 - Article
C2 - 18329245
AN - SCOPUS:42049119385
VL - 91
SP - 451
EP - 457
JO - Genomics
JF - Genomics
SN - 0888-7543
IS - 5
ER -