TY - JOUR
T1 - ZFAT is essential for endothelial cell assembly and the branch point formation of capillary-like structures in an angiogenesis model
AU - Yoshida, Yasuhiro
AU - Tsunoda, Toshiyuki
AU - Takashima, Yasuo
AU - Fujimoto, Takahiro
AU - Doi, Keiko
AU - Sasazuki, Takehiko
AU - Kuroki, Masahide
AU - Iwasaki, Akinori
AU - Shirasawa, Senji
N1 - Funding Information:
Acknowledgements.The authors would like to thank Takami Danno for her technical assistance. This study was supported by a grant from the “Genome Network Project” from the Ministry of Education, Culture, Sports, Science and Technology of Japan, and a Grant-in-Aid for Scientific Research (B) from the Japan Society for the Promotion of Science.
PY - 2010
Y1 - 2010
N2 - ZFAT, originally identified as a susceptibility gene for autoimmune thyroid disease, encodes a transcriptional regulator with one AT-hook and 18 C2H2-type zinc-finger domains. It is highly conserved among species. Here, we demonstrate that ZFAT is clearly expressed in human umbilical vein endothelial cells (HUVECs). Furthermore, we show that endothelial cell assembly and the branch point formation of capillary-like structures in HUVECs is impaired by the reduction of ZFAT expression through the use of ZFAT-miRNAs, whereas differences in cell proliferation or apoptotic features were not observed after the reduction in ZFAT expression. These results suggest that ZFAT may have critical roles in the capillary-like network formation that is involved in vascular remodeling. Elucidating the ZFAT-mediated transcriptional network will lead to a better understanding of the molecular mechanisms of angiogenesis.
AB - ZFAT, originally identified as a susceptibility gene for autoimmune thyroid disease, encodes a transcriptional regulator with one AT-hook and 18 C2H2-type zinc-finger domains. It is highly conserved among species. Here, we demonstrate that ZFAT is clearly expressed in human umbilical vein endothelial cells (HUVECs). Furthermore, we show that endothelial cell assembly and the branch point formation of capillary-like structures in HUVECs is impaired by the reduction of ZFAT expression through the use of ZFAT-miRNAs, whereas differences in cell proliferation or apoptotic features were not observed after the reduction in ZFAT expression. These results suggest that ZFAT may have critical roles in the capillary-like network formation that is involved in vascular remodeling. Elucidating the ZFAT-mediated transcriptional network will lead to a better understanding of the molecular mechanisms of angiogenesis.
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U2 - 10.2478/s11658-010-0028-y
DO - 10.2478/s11658-010-0028-y
M3 - Article
C2 - 20645017
AN - SCOPUS:78649687027
SN - 1425-8153
VL - 15
SP - 541
EP - 550
JO - Cellular and Molecular Biology Letters
JF - Cellular and Molecular Biology Letters
IS - 4
ER -
