ZFAT is essential for endothelial cell assembly and the branch point formation of capillary-like structures in an angiogenesis model

Yasuhiro Yoshida, Toshiyuki Tsunoda, Yasuo Takashima, Takahiro Fujimoto, Keiko Doi, Takehiko Sasazuki, Masahide Kuroki, Akinori Iwasaki, Senji Shirasawa

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

ZFAT, originally identified as a susceptibility gene for autoimmune thyroid disease, encodes a transcriptional regulator with one AT-hook and 18 C2H2-type zinc-finger domains. It is highly conserved among species. Here, we demonstrate that ZFAT is clearly expressed in human umbilical vein endothelial cells (HUVECs). Furthermore, we show that endothelial cell assembly and the branch point formation of capillary-like structures in HUVECs is impaired by the reduction of ZFAT expression through the use of ZFAT-miRNAs, whereas differences in cell proliferation or apoptotic features were not observed after the reduction in ZFAT expression. These results suggest that ZFAT may have critical roles in the capillary-like network formation that is involved in vascular remodeling. Elucidating the ZFAT-mediated transcriptional network will lead to a better understanding of the molecular mechanisms of angiogenesis.

Original languageEnglish
Pages (from-to)541-550
Number of pages10
JournalCellular and Molecular Biology Letters
Volume15
Issue number4
DOIs
Publication statusPublished - 2010

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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