Zirex: A novel zinc-regulated expression system for Lactococcus lactis

Dongdong Mu; Manuel Montalbán-López; Yoshimitsu Masuda; Oscar P. Kuipers

doi:10.1128/AEM.00866-13

Zirex: A novel zinc-regulated expression system for Lactococcus lactis

Dongdong Mu, Manuel Montalbán-López, Yoshimitsu Masuda, Oscar P. Kuipers

Research output: Contribution to journal › Article › peer-review

32 Citations (Scopus)

Abstract

Here, we report a new zinc-inducible expression system for Lactococcus lactis, called Zirex, consisting of the pneumococcal repressor SczA and P_czcD. P_czcD tightly regulates the expression of green fluorescent protein in L. lactis. We show the applicability of Zirex together with the nisin-controlled expression system, enabling simultaneous but independent regulation of different genes.

Original language	English
Pages (from-to)	4503-4508
Number of pages	6
Journal	Applied and environmental microbiology
Volume	79
Issue number	14
DOIs	https://doi.org/10.1128/AEM.00866-13
Publication status	Published - Jul 2013
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biotechnology
Food Science
Applied Microbiology and Biotechnology
Ecology

Access to Document

10.1128/AEM.00866-13

Cite this

@article{2ffdcd735a464abd8475d675f4a83809,

title = "Zirex: A novel zinc-regulated expression system for Lactococcus lactis",

abstract = "Here, we report a new zinc-inducible expression system for Lactococcus lactis, called Zirex, consisting of the pneumococcal repressor SczA and PczcD. PczcD tightly regulates the expression of green fluorescent protein in L. lactis. We show the applicability of Zirex together with the nisin-controlled expression system, enabling simultaneous but independent regulation of different genes.",

author = "Dongdong Mu and Manuel Montalb{\'a}n-L{\'o}pez and Yoshimitsu Masuda and Kuipers, {Oscar P.}",

year = "2013",

month = jul,

doi = "10.1128/AEM.00866-13",

language = "English",

volume = "79",

pages = "4503--4508",

journal = "Applied and Environmental Microbiology",

issn = "0099-2240",

publisher = "American Society for Microbiology",

number = "14",

}

TY - JOUR

T1 - Zirex

T2 - A novel zinc-regulated expression system for Lactococcus lactis

AU - Mu, Dongdong

AU - Montalbán-López, Manuel

AU - Masuda, Yoshimitsu

AU - Kuipers, Oscar P.

PY - 2013/7

Y1 - 2013/7

N2 - Here, we report a new zinc-inducible expression system for Lactococcus lactis, called Zirex, consisting of the pneumococcal repressor SczA and PczcD. PczcD tightly regulates the expression of green fluorescent protein in L. lactis. We show the applicability of Zirex together with the nisin-controlled expression system, enabling simultaneous but independent regulation of different genes.

AB - Here, we report a new zinc-inducible expression system for Lactococcus lactis, called Zirex, consisting of the pneumococcal repressor SczA and PczcD. PczcD tightly regulates the expression of green fluorescent protein in L. lactis. We show the applicability of Zirex together with the nisin-controlled expression system, enabling simultaneous but independent regulation of different genes.

UR - http://www.scopus.com/inward/record.url?scp=84879835274&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84879835274&partnerID=8YFLogxK

U2 - 10.1128/AEM.00866-13

DO - 10.1128/AEM.00866-13

M3 - Article

C2 - 23666339

AN - SCOPUS:84879835274

SN - 0099-2240

VL - 79

SP - 4503

EP - 4508

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 14

ER -

Zirex: A novel zinc-regulated expression system for Lactococcus lactis

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this