ZnO/SiO2core/shell nanowires for capturing CpG rich single-stranded DNAs

Marina Musa, Takao Yasui, Kazuki Nagashima, Masafumi Horiuchi, Zetao Zhu, Quanli Liu, Taisuke Shimada, Akihide Arima, Takeshi Yanagida, Yoshinobu Baba

Research output: Contribution to journalArticlepeer-review

Abstract

Atomic layer deposition (ALD) is capable of providing an ultrathin layer on high-aspect ratio structures with good conformality and tunable film properties. In this research, we modified the surface of ZnO nanowires through ALD for the fabrication of a ZnO/SiO2 (core/shell) nanowire microfluidic device which we utilized for the capture of CpG-rich single-stranded DNAs (ssDNA). Structural changes of the nanowires while varying the number of ALD cycles were evaluated by statistical analysis and their relationship with the capture efficiency was investigated. We hypothesized that finding the optimum number of ALD cycles would be crucial to ensure adequate coating for successful tuning to the desired surface properties, besides promoting a sufficient trapping region with optimal spacing size for capturing the ssDNAs as the biomolecules traverse through the dispersed nanowires. Using the optimal condition, we achieved high capture efficiency of ssDNAs (86.7%) which showed good potential to be further extended for the analysis of CpG sites in cancer-related genes. This finding is beneficial to the future design of core/shell nanowires for capturing ssDNAs in biomedical applications. This journal is

Original languageEnglish
Pages (from-to)337-344
Number of pages8
JournalAnalytical Methods
Volume13
Issue number3
DOIs
Publication statusPublished - Jan 21 2021

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Chemical Engineering(all)
  • Engineering(all)

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